LOCUS       sequence-106858-         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of HSPB3 (small HSP) tagged with V5 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Vos MJ, Zijlstra MP, Kanon B, van Waarde-Verhagen MA, Brunt ER, 
            Oosterveld-Hut HM, Carra S, Sibon OC, Kampinga HH
  TITLE     HSPB7 is the most potent polyQ aggregation suppressor within the 
            HSPB family of molecular chaperones.
  JOURNAL   Hum Mol Genet. 2010 Dec 1;19(23):4677-93. doi: 10.1093/hmg/ddq398. 
            Epub 2010 Sep 15.
  PUBMED    20843828
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/hmg/ddq398"; journalName: "Hum Mol Genet"; date: 
            "2010-12-1- 1"; volume: "19"; issue: "23"; pages: "4677-93"
COMMENT     Sequence Label: Frt-V5-hspB3
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    20..38
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     48..72
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             175..216
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(761..778)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 cctatcagtg atagagatct ccctatcagt gatagagatc gtcgacgagc tcgtttagtg
       61 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg
      121 gaccgatcca gcctccggac tctagcgttt aaacttaagc ttgaattcac catgggtaag
      181 cctatcccta accctctcct cggtctcgat tctacgggat ccaaaagcgg ccgcaccatg
      241 gcaaaaatca ttttgaggca cctcatagag attccagtgc gttaccagga agagtttgaa
      301 gctcgaggtc tagaagactg caggctggat catgctttat atgcactgcc tgggccaacc
      361 atcgtggacc tgaggaaaac cagggcagcg cagtctcctc cagtggactc agcggcagag
      421 acgccacccc gagaaggcaa atcccacttt cagatcctgc tggacgtggt ccagttcctc
      481 cctgaagaca tcatcattca gaccttcgaa ggctggctac tgataaaagc acaacacgga
      541 accagaatgg atgagcacgg ttttatctca agaagcttca cccgacagta caaactacca
      601 gatggcgtgg aaatcaaaga tttgtctgca gtcctctgtc atgatggaat tttggtggtg
      661 gaagtaaagg atccagttgg gactaagtga catcgtatcg gttcctgttc agaacgcgcc
      721 aaaatcgagt ctagagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt
      781 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
      841 cccactgtcc tttcctaata a
//
