LOCUS       sequence-106862-         799 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV 
            promoter and iRFP module. Compatible with MultiSite Gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, 
            Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, 
            Bode-Lesniewska B, Wild PJ, Frew IJ
  TITLE     A versatile modular vector system for rapid combinatorial mammalian 
            genetics.
  JOURNAL   J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
  PUBMED    25751063
REFERENCE   2  (bases 1 to 799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743."
COMMENT     Sequence Label: pMuLE ENTR CMV iRFP R4-R3
FEATURES             Location/Qualifiers
     source          1..799
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      106..115
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gcttggtacc gagctcggat ctgccgccac catggcggaa
      121 ggatccgtcg ccaggcagcc tgacctcttg acctgcgacg atgagccgat ccatatcccc
      181 ggtgccatcc aaccgcatgg actgctgctc gccctcgccg ccgacatgac gatcgttgcc
      241 ggcagcgaca accttcccga actcaccgga ctggcgatcg gcgccctgat cggccgctct
      301 gcggccgatg tcttcgactc ggagacgcac aaccgtctga cgatcgcctt ggccgagccc
      361 ggggcggccg tcggagcacc gatcactgtc ggcttcacga tgcgaaagga cgcaggcttc
      421 atcggctcct ggcatcgcca tgatcagctc atcttcctcg agctcgagcc tccccagcgg
      481 gacgtcgccg agccgcaggc gttcttccgc cgcaccaaca gcgccatccg ccgcctgcag
      541 gccgccgaaa ccttggaaag cgcctgcgcc gccgcggcgc aagaggtgcg gaagattacc
      601 ggcttcgatc gggtgatgat ctatcgcttc gcctccgact tcagcggcga agtgatcgca
      661 gaggatcggt gcgccgaggt cgagtcaaaa ctaggcctgc actatcctgc ctcaaccgtg
      721 ccggcgcagg cccgtcggct ctataccatc aacccggtac ggatcattcc cgatatcaat
      781 tatcggccgg tgccggtca
//
