LOCUS       sequence-106864-         646 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV 
            promoter and EGFP module. Compatible with MultiSite Gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 646)
  AUTHORS   Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, 
            Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, 
            Bode-Lesniewska B, Wild PJ, Frew IJ
  TITLE     A versatile modular vector system for rapid combinatorial mammalian 
            genetics.
  JOURNAL   J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
  PUBMED    25751063
REFERENCE   2  (bases 1 to 646)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743."
COMMENT     Sequence Label: pMuLE ENTR CMV eGFP L3-L2
FEATURES             Location/Qualifiers
     source          1..646
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(43..62)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(44..62)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(107..310)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(136..156)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        311..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 atggtggctt taggatccga gctcggtacc aagcttgggt ctccctatag tgagtcgtat
       61 taatttcgat aagccagtaa gcagtgggtt ctctagttag ccagagagct ctgcttatat
      121 agacctccca ccgtacacgc ctaccgccca tttgcgtcaa tggggcggag ttgttacgac
      181 attttggaaa gtcccgttga ttttggtgcc aaaacaaact cccattgacg tcaatggggt
      241 ggagacttgg aaatccccgt gagtcaaacc gctatccacg cccattgatg tactgccaaa
      301 accgcatcac catggtaata gcgatgacta atacgtagat gtactgccaa gtaggaaagt
      361 cccataaggt catgtactgg gcataatgcc aggcgggcca tttaccgtca ttgacgtcaa
      421 tagggggcgt acttggcata tgatacactt gatgtactgc caagtgggca gtttaccgta
      481 aatactccac ccattgacgt caatggaaag tccctattgg cgttactatg ggaacatacg
      541 tcattattga cgtcaatggg cgggggtcgt tgggcggtca gccaggcggg ccatttaccg
      601 taagttatgt aacgcggaac tccatatatg ggctatgaac taatga
//
