LOCUS       sequence-106866-         753 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MXS_chaining vector with PA-GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Sladitschek HL, Neveu PA
  TITLE     MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and 
            Flow Cytometry Approaches in Mammalian Systems.
  JOURNAL   PLoS One. 2015 Apr 24;10(4):e0124958. doi: 
            10.1371/journal.pone.0124958. eCollection 2015.
  PUBMED    25909630
REFERENCE   2  (bases 1 to 753)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-04-24"; volume: "10(4):e0124958. doi"; pages: " 
            10.1371/journal.pone.0124958. eCollection 2015"
COMMENT     Sequence Label: MXS_PA-GFP
FEATURES             Location/Qualifiers
     source          1..753
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     142..163
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        229..333
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(552..571)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
       61 cggccccgtg ctgctgcccg acaaccacta cctgagccac cagtccaagc tgagcaaaga
      121 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
      181 tctcggcatg gacgagctgt acaaggtcga ccaattcggg gaaatgtgcg cggaacccct
      241 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
      301 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
      361 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
      421 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
      481 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
      541 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
      601 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
      661 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
      721 aacactgcgg ccaacttact tctgacaacg atc
//
