LOCUS       sequence-106877-         618 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral plasmid for tet-inducible expression of mouse Oct4, Sox2,
            Klf4 and Myc for iPS cell generation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 618)
  AUTHORS   Carey BW, Markoulaki S, Hanna J, Saha K, Gao Q, Mitalipova M, 
            Jaenisch R
  TITLE     Reprogramming of murine and human somatic cells using a single 
            polycistronic vector.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Jan 6. 106(1):157-62.
  PUBMED    19109433
REFERENCE   2  (bases 1 to 618)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2009 Jan 6. 106(1):157-62."
COMMENT     Sequence Label: TetO-FUW-OSKM
FEATURES             Location/Qualifiers
     source          1..618
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    join(85..103,104..126,127..145,146..168,169..187,188..210,
                     211..229,230..252,253..271,272..294,295..313,314..336,
                     337..355)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        388..426
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     423..447
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca gagatccagt
       61 ttggttagat ctcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt
      121 taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg
      181 atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
      241 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta
      301 tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt
      361 gaaagtcgag ctcggtaccc gggtcgaggt aggcgtgtac ggtgggaggc ctatataagc
      421 agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc
      481 catagaagac accgggaccg atccagcctc cgcggccccg aattcgccat ggctggacac
      541 ctggcttcag acttcgcctt ctcaccccca ccaggtgggg gtgatgggtc agcagggctg
      601 gagccgggct gggtggat
//
