LOCUS       sequence-106887-         855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of HSPB8 (small HSP) tagged with V5 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Vos MJ, Zijlstra MP, Kanon B, van Waarde-Verhagen MA, Brunt ER, 
            Oosterveld-Hut HM, Carra S, Sibon OC, Kampinga HH
  TITLE     HSPB7 is the most potent polyQ aggregation suppressor within the 
            HSPB family of molecular chaperones.
  JOURNAL   Hum Mol Genet. 2010 Dec 1;19(23):4677-93. doi: 10.1093/hmg/ddq398. 
            Epub 2010 Sep 15.
  PUBMED    20843828
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/hmg/ddq398"; journalName: "Hum Mol Genet"; date: 
            "2010-12-1- 1"; volume: "19"; issue: "23"; pages: "4677-93"
COMMENT     Sequence Label: Frt-V5-hspB8
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    20..38
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     48..72
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             175..216
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 cctatcagtg atagagatct ccctatcagt gatagagatc gtcgacgagc tcgtttagtg
       61 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg
      121 gaccgatcca gcctccggac tctagcgttt aaacttaagc ttgaattcac catgggtaag
      181 cctatcccta accctctcct cggtctcgat tctacgggat ccaaaacctc gagaaccatg
      241 gctgacggtc agatgccctt ctcctgccac tacccaagcc gcctgcgccg agaccccttc
      301 cgggactctc ccctctcctc tcgcctgctg gatgatggct ttggcatgga ccccttccca
      361 gacgacttga cagcctcttg gcccgactgg gctctgcctc gtctctcctc cgcctggcca
      421 ggcaccctaa ggtcgggcat ggtgccccgg ggccccactg ccaccgccag gtttggggtg
      481 cctgccgagg gcaggacccc cccacccttc cctggggagc cctggaaagt gtgtgtgaat
      541 gtgcacagct tcaagccaga ggagttgatg gtgaagacca aagatggata cgtggaggtg
      601 tctggcaaac atgaagagaa acagcaagaa ggtggcattg tttctaagaa cttcacaaag
      661 aaaatccagc ttcctgcaga ggtggatcct gtgacagtat ttgcctcact ttccccagag
      721 ggtctgctga tcatcgaagc tccccaggtc cctccttact caacatttgg agagagcagt
      781 ttcaacaacg agcttcccca ggacagccag gaagtcacct gtacctgaga tgccagtact
      841 ggcccatcct tgttt
//
