LOCUS       sequence-106892-         855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of HSPB4 (small HSP) tagged with V5 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Vos MJ, Zijlstra MP, Kanon B, van Waarde-Verhagen MA, Brunt ER, 
            Oosterveld-Hut HM, Carra S, Sibon OC, Kampinga HH
  TITLE     HSPB7 is the most potent polyQ aggregation suppressor within the 
            HSPB family of molecular chaperones.
  JOURNAL   Hum Mol Genet. 2010 Dec 1;19(23):4677-93. doi: 10.1093/hmg/ddq398. 
            Epub 2010 Sep 15.
  PUBMED    20843828
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/hmg/ddq398"; journalName: "Hum Mol Genet"; date: 
            "2010-12-1- 1"; volume: "19"; issue: "23"; pages: "4677-93"
COMMENT     Sequence Label: Frt-V5-hspB4
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    11..29
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     39..63
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             166..207
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(814..831)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 gatagagatc tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag
       61 atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc
      121 agcctccgga ctctagcgtt taaacttaag cttgaattca ccatgggtaa gcctatccct
      181 aaccctctcc tcggtctcga ttctacggga tccaaaacct cgagaaccat ggacgtgacc
      241 atccagcacc cctggttcaa gcgcaccctg gggcccttct accccagccg gctgttcgac
      301 cagtttttcg gcgagggcct ttttgagtat gacctgctgc ccttcctgtc gtccaccatc
      361 agcccctact accgccagtc cctcttccgc accgtgctgg actccggcat ctctgaggtt
      421 cgatccgacc gggacaagtt cgtcatcttc ctcgatgtga agcacttctc cccggaggac
      481 ctcaccgtga aggtgcagga cgactttgtg gagatccacg gaaagcacaa cgagcgccag
      541 gacgaccacg gctacatttc ccgtgagttc caccgccgct accgcctgcc gtccaacgtg
      601 gaccagtcgg ccctctcttg ctccctgtct gccgatggca tgctgacctt ctgtggcccc
      661 aagatccaga ctggcctgga tgccacccac gccgagcgag ccatccccgt gtcgcgggag
      721 gagaagccca cctcggctcc ctcgtcctaa gcaggcattg cctcggacgc gccaaaatcg
      781 agtctagagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc
      841 catctgttgt ttgcc
//
