LOCUS       sequence-106906-         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of hUb in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  AUTHORS   VanderLinden RT, Hemmis CW, Schmitt B, Ndoja A, Whitby FG, Robinson 
            H, Cohen RE, Yao T, Hill CP
  TITLE     Structural Basis for the Activation and Inhibition of the UCH37 
            Deubiquitylase.
  JOURNAL   Mol Cell. 2015 Feb 17. pii: S1097-2765(15)00017-9. doi: 
            10.1016/j.molcel.2015.01.016.
  PUBMED    25702872
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2015 Feb 17. pii: S1097-2765(15)00017-9. doi: 
            10.1016/j.molcel.2015.01.016."
COMMENT     Sequence Label: pET3a-hUb
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             33..260
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     primer_bind     complement(314..332)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      328..375
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(741..746,747..763,764..769))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(796..814)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tattttgttt actttaagaa ggagatatac atatgcagat cttcgtcaag acgttaaccg
       61 gtaaaaccat aactctagaa gttgaaccat ccgataccat cgaaaacgtt aaggctaaaa
      121 ttcaagacaa ggaaggcatt ccacctgatc aacaaagatt gatctttgcc ggtaagcagc
      181 tcgaggacgg tagaacgctg tctgattaca acattcagaa ggagtcgacc ttacatcttg
      241 tcttaagact aagaggtggt tgaggatccg gctgctaaca aagcccgaaa ggaagctgag
      301 ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc
      361 ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt
      421 cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg
      481 gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata
      541 tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa
      601 gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc
      661 gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt
      721 taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt
      781 cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
      841 tggtttctta gacgtcaggt ggcactt
//
