LOCUS       sequence-106916-         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of hUCH37 (ISF1) in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   VanderLinden RT, Hemmis CW, Schmitt B, Ndoja A, Whitby FG, Robinson 
            H, Cohen RE, Yao T, Hill CP
  TITLE     Structural Basis for the Activation and Inhibition of the UCH37 
            Deubiquitylase.
  JOURNAL   Mol Cell. 2015 Feb 17. pii: S1097-2765(15)00017-9. doi: 
            10.1016/j.molcel.2015.01.016.
  PUBMED    25702872
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2015 Feb 17. pii: S1097-2765(15)00017-9. doi: 
            10.1016/j.molcel.2015.01.016."
COMMENT     Sequence Label: pET151-hUCH37 (ISF1)
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             26..43
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             44..85
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             86..106
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 actttaagaa ggagatatac atatgcatca tcaccatcac catggtaagc ctatccctaa
       61 ccctctcctc ggtctcgatt ctacggaaaa cctgtatttt cagggaattg atcccttcac
      121 catgacgggc aatgccgggg agtggtgcct catggaaagc gaccccgggg tcttcaccga
      181 gctcattaaa ggattcggtt gccgaggagc ccaagtagaa gaaatatgga gtttagagcc
      241 tgagaatttt gaaaaattaa agccagttca tgggttaatt tttcttttca agtggcagcc
      301 aggagaagaa ccagcaggct ctgtggttca ggactcccga cttgacacga tattttttgc
      361 taagcaggta attaataatg cttgtgctac tcaagccata gtgagtgtgt tactgaactg
      421 tacccaccag gatgtccatt taggcgagac attatcagag tttaaagaat tttcacaaag
      481 ttttgatgca gctatgaaag gcttggcact gagcaattca gatgtgattc gacaagtaca
      541 caacagtttc gccagacagc aaatgtttga atttgatacg aagacatcag caaaagaaga
      601 agatgctttt cactttgtca gttatgttcc tgttaatggg agactgtatg aattagatgg
      661 attaagagaa ggaccgattg atttaggtgc atgcaatcaa gatgattgga tcagtgcagt
      721 aaggcctgtc atagaaaaaa ggatacaaaa gtacagtgaa ggtgaaattc gatttaattt
      781 aatggccatt gtgtctgaca gaaaaatgat atatgagcag aagatagcag agttacaaag
      841 acaacttgca gaggaggaac ccatggatac agatcaaggt aatagtatgt taagtgctat
//
