LOCUS       sequence-106919-         744 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  splicing reporter (with intron) with elements to shorten the 
            half-life of the luciferase protein as well as the luciferase mRNA..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Younis I, Berg M, Kaida D, Dittmar K, Wang C, Dreyfuss G
  TITLE     Rapid-response splicing reporter screens identify differential 
            regulators of constitutive and alternative splicing.
  JOURNAL   Mol Cell Biol. 2010 Apr;30(7):1718-28. doi: 10.1128/MCB.01301-09. 
            Epub 2010 Feb 1.
  PUBMED    20123975
REFERENCE   2  (bases 1 to 744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.01301-09"; journalName: "Mol Cell Biol"; date: 
            "2010-04"; volume: "30"; issue: "7"; pages: "1718-28"
COMMENT     Sequence Label: CMV-LUC2CP/intron/ARE
FEATURES             Location/Qualifiers
     source          1..744
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        183..540
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        183..374
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(206..226)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      391..526
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     453..472
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
ORIGIN
        1 ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta
       61 aaacctctac aaatgtggta aaatcgataa ggatccgttt gcgtattggg cgctcttccg
      121 ctgatctgcg cagcaccatg gcctgaaata acctctgaaa gaggaacttg gttagctacc
      181 ttctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
      241 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
      301 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
      361 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
      421 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct
      481 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
      541 aagctcgatt cttctgacac tagcgccacc atgaagaagc ccgaactcac cgctaccagc
      601 gttgaaaaat ttctcatcga gaagttcgac agtgtgagcg acctgatgca gttgtcggag
      661 ggcgaagaga gccgagcctt cagcttcgat gtcggcggac gcggctatgt actgcgggtg
      721 aatagctgcg ctgatggctt ctac
//
