LOCUS       sequence-106920-         877 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of HSPB10 (small HSP) tagged with V5 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Vos MJ, Zijlstra MP, Kanon B, van Waarde-Verhagen MA, Brunt ER, 
            Oosterveld-Hut HM, Carra S, Sibon OC, Kampinga HH
  TITLE     HSPB7 is the most potent polyQ aggregation suppressor within the 
            HSPB family of molecular chaperones.
  JOURNAL   Hum Mol Genet. 2010 Dec 1;19(23):4677-93. doi: 10.1093/hmg/ddq398. 
            Epub 2010 Sep 15.
  PUBMED    20843828
REFERENCE   2  (bases 1 to 877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/hmg/ddq398"; journalName: "Hum Mol Genet"; date: 
            "2010-12-1- 1"; volume: "19"; issue: "23"; pages: "4677-93"
COMMENT     Sequence Label: Frt-V5-hspB10
FEATURES             Location/Qualifiers
     source          1..877
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    11..29
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     39..63
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             166..207
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 gatagagatc tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag
       61 atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc
      121 agcctccgga ctctagcgtt taaacttaag cttgaattca ccatgggtaa gcctatccct
      181 aaccctctcc tcggtctcga ttctacggga tccaaaacct cgagaaccat ggctgcactg
      241 agttgtctct tggacagtgt cagaagggac ataaagaagg tggacagaga actaaggcaa
      301 ctgagatgca tcgacgaatt tagcacacgg tgcctgtgcg acttgtatat gcacccctat
      361 tgctgctgtg acttgcaccc atatccgtac tgcttgtgct attccaagcg atcacgctct
      421 tgcggcctgt gtgatctcta cccatgttgc ctgtgtgatt ataagcttta ctgtctgcga
      481 ccatctctca gaagtttgga gaggaaagcc atcagagcca tagaagatga gaagcgagag
      541 cttgccaaac tgagaagaac aacaaataga attctggctt cctcctgctg tagcagtaac
      601 attttaggat cggtgaatgt atgcggtttt gaacccgatc aagtcaaagt tcgagtgaag
      661 gatggaaagg tatgtgtgtc ggctgagcgg gagaacaggt acgactgcct tggatcgaaa
      721 aagtacagct acatgaacat ctgcaaagag ttcagcttgc cgccctgtgt ggatgagaag
      781 gatgtaacat actcctatgg gctcggcagc tgtgtcaaga tcgagtctcc ttgctaccct
      841 tgcacttctc cttgcagccc gtgcagcccg tgcaacc
//
