LOCUS       sequence-106926-         744 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral expression of EGFP-TEV-S-tag CENP-O for protein 
            localization and affinity purification (LAP).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 744)
  TITLE     Cheeseman Lab Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pIC145
FEATURES             Location/Qualifiers
     source          1..744
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             36..56
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             69..113
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 atcggtggag ctccaccggt ggcggccgct ctagagagaa tctttatttt cagggcgccg
       61 ccaaattcaa agaaaccgct gctgctaaat tcgaacgcca gcacatggac agcggaggtg
      121 gaggtactag tctcgagatg gagcaggcga accctttacg tccagatggc gagtccaaag
      181 gaggtgtttt agctcacttg gaaaggctag agacccaagt gagcagatcc cgtaaacagt
      241 ctgaagagct gcagagcgtg caggcccagg aaggtgctct tggaaccaag attcataaac
      301 taaggcgtct gcgagatgag ctgagggctg tggtgcggca ccggcgagcc agcgtgaaag
      361 catgtattgc caatgtagaa cccaaccaaa cagtggagat caatgagcaa gaagcattgg
      421 aagagaaatt ggaaaatgtg aaagccattc tgcaggcata tcattttaca ggcctcagtg
      481 gtaaactgac cagccgagga gtttgtgtct gcatcagtac tgcttttgag gggaacctat
      541 tggattccta ttttgtggac cttgtcatac agaaaccact ccggatacat caccattcag
      601 tcccagtctt cattcccctg gaagagatag ctgcaaaata tttacagacc aacatccagc
      661 acttcctgtt cagtctctgc gagtacctga atgcttactc tgggaggaag taccaggcag
      721 accggcttca gagtgacttt gcag
//
