LOCUS       sequence-106929-         811 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of HSPB1 (small HSP) tagged with V5 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Vos MJ, Zijlstra MP, Kanon B, van Waarde-Verhagen MA, Brunt ER, 
            Oosterveld-Hut HM, Carra S, Sibon OC, Kampinga HH
  TITLE     HSPB7 is the most potent polyQ aggregation suppressor within the 
            HSPB family of molecular chaperones.
  JOURNAL   Hum Mol Genet. 2010 Dec 1;19(23):4677-93. doi: 10.1093/hmg/ddq398. 
            Epub 2010 Sep 15.
  PUBMED    20843828
REFERENCE   2  (bases 1 to 811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/hmg/ddq398"; journalName: "Hum Mol Genet"; date: 
            "2010-12-1- 1"; volume: "19"; issue: "23"; pages: "4677-93"
COMMENT     Sequence Label: Frt-V5-hspB1
FEATURES             Location/Qualifiers
     source          1..811
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    18..36
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     46..70
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             173..214
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 tatcagtgat agagatctcc ctatcagtga tagagatcgt cgacgagctc gtttagtgaa
       61 ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggga
      121 ccgatccagc ctccggactc tagcgtttaa acttaagctt gaattcacca tgggtaagcc
      181 tatccctaac cctctcctcg gtctcgattc tacgggatcc aaaacctcga gaagcatgac
      241 cgagcgccgc gtccccttct cgctcctgcg gggccccagc tgggacccct tccgcgactg
      301 gtacccgcat agccgcctct tcgaccaggc cttcgggctg ccccggctgc cggaggagtg
      361 gtcgcagtgg ttaggcggca gcagctggcc aggctacgtg cgccccctgc cccccgccgc
      421 catcgagagc cccgcagtgg ccgcgcccgc ctacagccgc gcgctcagcc ggcaactcag
      481 cagcggggtc tcggagatcc ggcacactgc ggaccgctgg cgcgtgtccc tggatgtcaa
      541 ccacttcgcc ccggacgagc tgacggtcaa gaccaaggat ggcgtggtgg agatcaccgg
      601 caagcacgag gagcggcagg acgagcatgg ctacatctcc cggtgcttca cgcggaaata
      661 cacgctgccc cccggtgtgg accccaccca agtttcctcc tccctgtccc ctgagggcac
      721 actgaccgtg gaggccccca tgcccaagct agccacgcag tccaacgaga tcaccatccc
      781 agtcaccttc gagtcgcggg cccagcttgg g
//
