LOCUS       sequence-106930-         767 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of hUCH37 (ISF2) in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 767)
  AUTHORS   VanderLinden RT, Hemmis CW, Schmitt B, Ndoja A, Whitby FG, Robinson 
            H, Cohen RE, Yao T, Hill CP
  TITLE     Structural Basis for the Activation and Inhibition of the UCH37 
            Deubiquitylase.
  JOURNAL   Mol Cell. 2015 Feb 17. pii: S1097-2765(15)00017-9. doi: 
            10.1016/j.molcel.2015.01.016.
  PUBMED    25702872
REFERENCE   2  (bases 1 to 767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2015 Feb 17. pii: S1097-2765(15)00017-9. doi: 
            10.1016/j.molcel.2015.01.016."
COMMENT     Sequence Label: pET151-hUCH37 (ISF2)
FEATURES             Location/Qualifiers
     source          1..767
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             35..52
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             53..94
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             95..115
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 attttgttta ctttaagaag gagatataca tatgcatcat caccatcacc atggtaagcc
       61 tatccctaac cctctcctcg gtctcgattc tacggaaaac ctgtattttc agggaattga
      121 tcccttcacc atgacgggca atgccgggga gtggtgcctc atggaaagcg accccggggt
      181 cttcaccgag ctcattaaag gattcggttg ccgaggagcc caagtagaag aaatatggag
      241 tttagagcct gagaattttg aaaaattaaa gccagttcat gggttaattt ttcttttcaa
      301 gtggcagcca ggagaagaac cagcaggctc tgtggttcag gactcccgac ttgacacgat
      361 attttttgct aagcaggtaa ttaataatgc ttgtgctact caagccatag tgagtgtgtt
      421 actgaactgt acccaccagg atgtccattt aggcgagaca ttatcagagt ttaaagaatt
      481 ttcacaaagt tttgatgcag ctatgaaagg cttggcactg agcaattcag atgtgattcg
      541 acaagtacac aacagtttcg ccagacagca aatgtttgaa tttgatacga agacatcagc
      601 aaaagaagaa gatgcttttc actttgtcag ttatgttcct gttaatggga gactgtatga
      661 attagatgga ttaagagaag gaccgattga tttaggtgca tgcaatcaag atgattggat
      721 cagtgcagta aggcctgtca tagaaaaaag gatacaaaag tacagtg
//
