LOCUS       sequence-10694-e        2316 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Johannessen CM, Boehm JS, Kim SY, Thomas SR, Wardwell L, Johnson LA,
            Emery CM, Stransky N, Cogdill AP, Barretina J, Caponigro G, 
            Hieronymus H, Murray RR, Salehi-Ashtiani K, Hill DE, Vidal M, Zhao 
            JJ, Yang X, Alkan O, Kim S, Harris JL, Wilson CJ, Myer VE, Finan PM,
            Root DE, Roberts TM, Golub T, Flaherty KT, Dummer R, Weber BL, 
            Sellers WR, Schlegel R, Wargo JA, Hahn WC, Garraway LA
  TITLE     COT drives resistance to RAF inhibition through MAP kinase pathway 
            reactivation.
  JOURNAL   Nature. 2010 Dec 16;468(7326):968-72. doi: 10.1038/nature09627
  PUBMED    21107320
REFERENCE   2  (bases 1 to 2316)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-12-16- 16"; volume: "468"; issue: "7326"; 
            pages: "968-72"
COMMENT     Sequence Label: pDONR223-TLK2
FEATURES             Location/Qualifiers
     source          1..2316
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(579..590)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 atggaagaat tgcatagcct ggacccacga cggcaggaat tattggaggc caggtttact
       61 ggagtaggtg ttagtaaggg accacttaat agtgagtctt ccaaccagag cttgtgcagc
      121 gtcggatcct tgagtgataa agaagtagag actcccgaga aaaagcagaa tgaccagcga
      181 aatcggaaaa gaaaagctga accatatgaa actagccaag ggaaaggcac tcctagggga
      241 cataaaatta gtgattactt tgagtttgct gggggaagcg cgccaggaac cagccctggc
      301 agaagtgttc caccagttgc acgatcctca ccgcaacatt ccttatccaa tcccttaccg
      361 cgacgagtag aacagcccct ctatggttta gatggcagtg ctgcaaagga ggcaacggag
      421 gagcagtctg ctctgccaac cctcatgtca gtgatgctag caaaacctcg gcttgacaca
      481 gagcagctgg cgcaaagggg agctggcctc tgcttcactt ttgtttcagc tcagcaaaac
      541 agtccctcat ctacgggatc tggcaacaca gagcattcct gcagctccca aaaacagatc
      601 tccatccagc acagacagac ccagtccgac ctcacaatag aaaaaatatc tgcactagaa
      661 aacagtaaga attctgactt agagaagaag gagggaagaa tagatgattt attaagagcc
      721 aactgtgatt tgagacggca gattgatgaa cagcaaaaga tgctagagaa atacaaggaa
      781 cgattaaata gatgtgtgac aatgagcaag aaactcctta tagaaaagtc aaaacaagag
      841 aagatggcgt gtagagataa gagcatgcaa gaccgcttga gactgggcca ctttactact
      901 gtccgacacg gagcctcatt tactgaacag tggacagatg gttatgcttt tcagaatctt
      961 atcaagcaac aggaaaggat aaattcacag agggaagaga tagaaagaca acggaaaatg
     1021 ttagcaaagc ggaaacctcc tgccatgggt caggcccctc ctgcaaccaa tgagcagaaa
     1081 cagcggaaaa gcaagaccaa tggagctgaa aatgaaacgc cctcttctgg gaatacagag
     1141 ctaaaggata cagccccagc cttaggagcc cacagtttac ttaggttaac gttagcagaa
     1201 taccatgaac aagaagaaat cttcaaactc agattaggtc atcttaaaaa ggaggaagca
     1261 gagatccagg cagagctgga gagactagaa agggttagaa atctacatat cagggaacta
     1321 aaaaggatac ataatgaaga taattcacaa tttaaagatc atccaacgct aaatgacaga
     1381 tatttgttgt tacatctttt gggtagagga ggtttcagtg aagtttacaa ggcatttgat
     1441 ctaacagagc aaagatacgt agctgtgaaa attcaccagt taaataaaaa ctggagagat
     1501 gagaaaaagg agaattacca caagcatgca tgtagggaat accggattca taaagagctg
     1561 gatcatccca gaatagttaa gctgtatgat tacttttcac tggatactga ctcgttttgt
     1621 acagtattag aatactgtga gggaaatgat ctggacttct acctgaaaca gcacaaatta
     1681 atgtcggaga aagaggcccg gtccattatc atgcagattg tgaatgcttt aaagtactta
     1741 aatgaaataa aacctcccat catacactat gacctcaaac caggtaatat tcttttagta
     1801 aatggtacag cgtgtggaga gataaaaatt acagattttg gtctttcgaa gatcatggat
     1861 gatgatagct acaattcagt ggatggcatg gagctaacat cacaaggtgc tggtacttat
     1921 tggtatttac caccagagtg ttttgtggtt gggaaagaac caccaaagat ctcaaataaa
     1981 gttgatgtgt ggtcggtggg tgtgatcttc tatcagtgtc tttatggaag gaagcctttt
     2041 ggccataacc agtctcagca agacatccta caagagaata cgattcttaa agctactgaa
     2101 gtgcagttcc cgccaaagcc agtagtaaca cctgaagcaa aggcgtttat tcgacgatgc
     2161 ttggcctacc gaaaggagga ccgcattgat gtccagcagc tggcctgtga tccctacttg
     2221 ttgcctcaca tccgaaagtc agtctctaca agtagccctg ctggagctgc tattgcatca
     2281 acctctgggg cgtccaataa cagttcttct aattga
//
