LOCUS       sequence-106950-         709 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral expression of EGFP-TEV-S-tag CENP-P for protein 
            localization and affinity purification (LAP).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 709)
  TITLE     Cheeseman Lab Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 709)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pIC146
FEATURES             Location/Qualifiers
     source          1..709
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             47..67
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             80..124
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 gtccggaggg gatcggtgga gctccaccgg tggcggccgc tctagagaga atctttattt
       61 tcagggcgcc gccaaattca aagaaaccgc tgctgctaaa ttcgaacgcc agcacatgga
      121 cagcggaggt ggaggtacta gtctcgagat ggacgcagag ctggcagagg tgcgcgcctt
      181 gcaagctgag atcgcggccc tgcggcgagc gtgtgaggac ccaccggcgc cctgggaaga
      241 gaagtcccga gtccaaaaat cttttcaagc catacaccaa ttcaatttgg aaggatggaa
      301 gtcttcaaaa gatctgaaaa atcagcttgg acatttagaa tcagaacttt catttctaag
      361 tacgcttact ggcatcaata taagaaatca ctccaagcag acagaagacc taacaagcac
      421 tgagatgaca gaaaagagta ttagaaaagt tctacagaga cacagattat caggaaattg
      481 ccacatggtt acatttcaac ttgaatttca gattctggaa attcagaata aggagagatt
      541 atcttctgct gttactgacc tcaacataat aatggagccc acagaatgct cagaattaag
      601 tgaatttgtg tctagagcag aagagagaaa agatctgttc atgtttttcc gaagcctgca
      661 tttttttgtg gagtggtttg aatatcgtaa gcgcacgttt aaacatctc
//
