LOCUS       sequence-106955-         902 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of HSPB3 (small HSP) tagged with V5 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Vos MJ, Zijlstra MP, Kanon B, van Waarde-Verhagen MA, Brunt ER, 
            Oosterveld-Hut HM, Carra S, Sibon OC, Kampinga HH
  TITLE     HSPB7 is the most potent polyQ aggregation suppressor within the 
            HSPB family of molecular chaperones.
  JOURNAL   Hum Mol Genet. 2010 Dec 1;19(23):4677-93. doi: 10.1093/hmg/ddq398. 
            Epub 2010 Sep 15.
  PUBMED    20843828
REFERENCE   2  (bases 1 to 902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/hmg/ddq398"; journalName: "Hum Mol Genet"; date: 
            "2010-12-1- 1"; volume: "19"; issue: "23"; pages: "4677-93"
COMMENT     Sequence Label: Frt-V5-hspB3
FEATURES             Location/Qualifiers
     source          1..902
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(513..554)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(657..681)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    691..709
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    712..730
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        complement(732..831)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(761..781)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 tcgattttgg cgcgttctga acaggaaccg atacgatgtc acttagtccc aactggatcc
       61 tttacttcca ccaccaaaat tccatcatga cagaggactg cagacaaatc tttgatttcc
      121 acgccatctg gtagtttgta ctgtcgggtg aagcttcttg agataaaacc gtgctcatcc
      181 attctggttc cgtgttgtgc ttttatcagt agccagcctt cgaaggtctg aatgatgatg
      241 tcttcaggga ggaactggac cacgtccagc aggatctgaa agtgggattt gccttctcgg
      301 ggtggcgtct ctgccgctga gtccactgga ggagactgcg ctgccctggt tttcctcagg
      361 tccacgatgg ttggcccagg cagtgcatat aaagcatgat ccagcctgca gtcttctaga
      421 cctcgagctt caaactcttc ctggtaacgc actggaatct ctatgaggtg cctcaaaatg
      481 atttttgcca tggtgcggcc gcttttggat cccgtagaat cgagaccgag gagagggtta
      541 gggataggct tacccatggt gaattcaagc ttaagtttaa acgctagagt ccggaggctg
      601 gatcggtccc ggtgtcttct atggaggtca aaacagcgtg gatggcgtct ccaggcgatc
      661 tgacggttca ctaaacgagc tcgtcgacga tctctatcac tgatagggag atctctatca
      721 ctgataggga gagctctgct tatatagacc tcccaccgta cacgcctacc gcccatttgc
      781 gtcaatgggg cggagttgtt acgacatttt ggaaagtccc gttgattttg gtgccaaaac
      841 aaactcccat tgacgtcaat ggggtggaga cttggaaatc cccgtgagtc aaaccgctat
      901 cc
//
