LOCUS       sequence-106966-         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of HSPB7 (small HSP) tagged with V5 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Vos MJ, Zijlstra MP, Kanon B, van Waarde-Verhagen MA, Brunt ER, 
            Oosterveld-Hut HM, Carra S, Sibon OC, Kampinga HH
  TITLE     HSPB7 is the most potent polyQ aggregation suppressor within the 
            HSPB family of molecular chaperones.
  JOURNAL   Hum Mol Genet. 2010 Dec 1;19(23):4677-93. doi: 10.1093/hmg/ddq398. 
            Epub 2010 Sep 15.
  PUBMED    20843828
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/hmg/ddq398"; journalName: "Hum Mol Genet"; date: 
            "2010-12-1- 1"; volume: "19"; issue: "23"; pages: "4677-93"
COMMENT     Sequence Label: Frt-V5-hspB7
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(577..618)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(721..745)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    755..773
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    776..794
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        complement(796..847)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(825..845)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 gactcgattt tggcgcgtca gggaaaggga agggagaggc actcagattt tgatctccgt
       61 ccggaaggtc tgctggacgt gttctgtatg cgggtgacgc cgtgcccgga tagtgaggct
      121 gccgtcctcc cgcagagccg aggtcaccga cgtcgggtcc acgtcctccg gcagctggca
      181 cttgtgagcg aaggtgttca tgacggtgcc gtcagccgcc agcttctcag cccgcacctc
      241 gatgtggttg ttggaggtgg tgacaatgat gtcttcaggt gagaagtctc tcacgtccac
      301 cgcaaactca taggcgtctc ctagggtctt gatgttgcct gccccaccgg ggcgggctgg
      361 gaaggccagg ggctccgagt ggggccgcat gaagctgcca aagtcatcgg aaaacatgct
      421 cagggccttc tccatgggcg ggtcctgggc cgggagggca cgggaggccg aggaggaggt
      481 ggaagaggag gaggaagaag aagaggaatg gaaacttctc tccgctcgga aggtggaaga
      541 ggttctgtgg ctcatggttc tcgaggtttt ggatcccgta gaatcgagac cgaggagagg
      601 gttagggata ggcttaccca tggtgaattc aagcttaagt ttaaacgcta gagtccggag
      661 gctggatcgg tcccggtgtc ttctatggag gtcaaaacag cgtggatggc gtctccaggc
      721 gatctgacgg ttcactaaac gagctcgtcg acgatctcta tcactgatag ggagatctct
      781 atcactgata gggagagctc tgcttatata gacctcccac cgtacacgcc taccgcccat
      841 ttgcgtcaat gg
//
