LOCUS       sequence-106980-         790 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of hRPN13 (285-407) (Q337A/Q338A) in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 790)
  AUTHORS   VanderLinden RT, Hemmis CW, Schmitt B, Ndoja A, Whitby FG, Robinson 
            H, Cohen RE, Yao T, Hill CP
  TITLE     Structural Basis for the Activation and Inhibition of the UCH37 
            Deubiquitylase.
  JOURNAL   Mol Cell. 2015 Feb 17. pii: S1097-2765(15)00017-9. doi: 
            10.1016/j.molcel.2015.01.016.
  PUBMED    25702872
REFERENCE   2  (bases 1 to 790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2015 Feb 17. pii: S1097-2765(15)00017-9. doi: 
            10.1016/j.molcel.2015.01.016."
COMMENT     Sequence Label: pDEST15.1-hRPN13 (285-407) (Q337A/Q338A)
FEATURES             Location/Qualifiers
     source          1..790
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    30..54
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(466..486)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     protein_bind    complement(507..531)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(585..607)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
ORIGIN
        1 gttagcagcc ggatcccggg tcgaatcaaa ccactttgta caagaaagct gggtcggcgc
       61 gcccaccctt cgaggatcct attatcagtc caggctcatg tcctcctctt cgtccttctt
      121 gtccttcgtg tcgccctctt tctgctcggg cttggcgttg ttctgcatgg ctttggcaaa
      181 cgcttccaca tcgcccttgt tggcggcctc cacagcctct gcaggcagac cgaactggca
      241 catgaggggg cccagctgcc ccgaggccaa ggctgcgctg aacatgccca gggccgccgc
      301 gaactggggc gaggtcaggg tattctggat ctcatccgcg gtctgcggca gcgactcccc
      361 agatggcaag tagggaagca ggcgctcctg gacatccgcg ttggcgagga tgggagccat
      421 tatctccggc gtcagcacac tggccaggtc cacttgggtc caggagccct gaaaatacag
      481 gttttcctcg agtacggcgg ccgcggagcc tgcttttttg tacaaacttg tttgattcga
      541 ccatggacgc ggaaccagat ccgattttgg aggatggtcg ccaccaccaa acgtggcttg
      601 ccagccctgc aaaggccatg ctatatactt gctggatttc aagtacttat caatttgtgg
      661 gatagcttca atacgttttt taaaacaaac taattttggg aacgcatcca ggcacattgg
      721 gtccatgtat aaaacaacat caagagcgtc atacaacatg aagtcaggat gggttacatg
      781 atcaccattt
//
