LOCUS       sequence-106991-         708 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral expression of EGFP-TEV-S-tag CENP-A for protein 
            localization and affinity purification (LAP).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 708)
  TITLE     Cheeseman Lab Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pKG96
FEATURES             Location/Qualifiers
     source          1..708
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             57..77
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             90..134
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 agagctgtca gtccggaggg gatcggtgga gctccaccgg tggcggccgc tctagagaga
       61 atctttattt tcagggcgcc gccaaattca aagaaaccgc tgctgctaaa ttcgaacgcc
      121 agcacatgga cagcggaggt ggaggtacta gtctcgaggg cccgcgccgc cggagccgaa
      181 agcccgaggt cccgaggagg cgcagcccga gcccgacccc gacccccggc ccctcccggc
      241 ggggcccctc cttaggcgct tcctcccatc aacacagtcg gcggagacaa ggttggctaa
      301 aggagatccg aaagcttcag aagagcacac acctcttgat aaggaagctg cccttcagcc
      361 gcctggcaag agaaatatat gttaaattca ctcgtggtgt ggacttcaat tggcaagccc
      421 aggccctatt ggccctacaa gaggcagcag aagcatttct agttcatctc tttgaggacg
      481 cctatctcct caccttacat gcaggccgag ttactttctt cccaaaggat gtgcaactgg
      541 cccggaggat ccggggcctt gaggagggac tcggctgaga attctgcagt cgacggtacc
      601 gcgggcccgg gatccaccgg atctagataa ctgatcataa tcagccatac cacatttgta
      661 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctg
//
