LOCUS       sequence-106993-         793 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral expression of EGFP-TEV-S-tag CENP-A for protein 
            localization and affinity purification (LAP).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 793)
  TITLE     Cheeseman Lab Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pKG96
FEATURES             Location/Qualifiers
     source          1..793
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(592..636)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             complement(649..669)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(757..778)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa
       61 acctctacaa atgtggtatg gctgattatg atcagttatc tagatccggt ggatcccggg
      121 cccgcggtac cgtcgactgc agaattctca gccgagtccc tcctcaaggc cccggatcct
      181 ccgggccagt tgcacatcct ttgggaagaa agtaactcgg cctgcatgta aggtgaggag
      241 ataggcgtcc tcaaagagat gaactagaaa tgcttctgct gcctcttgta gggccaatag
      301 ggcctgggct tgccaattga agtccacacc acgagtgaat ttaacatata tttctcttgc
      361 caggcggctg aagggcagct tccttatcaa gaggtgtgtg ctcttctgaa gctttcggat
      421 ctcctttagc caaccttgtc tccgccgact gtgttgatgg gaggaagcgc ctaaggaggg
      481 gccccgccgg gaggggccgg gggtcggggt cgggctcggg ctgcgcctcc tcgggacctc
      541 gggctttcgg ctccggcggc gcgggccctc gagactagta cctccacctc cgctgtccat
      601 gtgctggcgt tcgaatttag cagcagcggt ttctttgaat ttggcggcgc cctgaaaata
      661 aagattctct ctagagcggc cgccaccggt ggagctccac cgatcccctc cggacttgta
      721 cagctcgtcc atgccgagag tgatcccggc ggcggtcacg aactccagca ggaccatgtg
      781 atcgcgcttc tcg
//
