LOCUS       sequence-107020-         818 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for RFP657 NHEJ reporter system expression. Allows
            insertion of target sites between the SFFV promoter and RFP657 for 
            detection of fluorescence knockdown. Puromycin resistance..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Heckl D, Kowalczyk MS, Yudovich D, Belizaire R, Puram RV, McConkey 
            ME, Thielke A, Aster JC, Regev A, Ebert BL
  TITLE     Generation of mouse models of myeloid malignancy with combinatorial 
            genetic lesions using CRISPR-Cas9 genome editing.
  JOURNAL   Nat Biotechnol. 2014 Jun 22. doi: 10.1038/nbt.2951.
  PUBMED    24952903
REFERENCE   2  (bases 1 to 818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2014 Jun 22. doi: 10.1038/nbt.2951."
COMMENT     Sequence Label: pL-SFFV.Reporter.RFP657.PAC
FEATURES             Location/Qualifiers
     source          1..818
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             90..788
                     /codon_start=1
                     /product="far-red monomeric fluorescent protein with an 
                     emission peak at 657 nm (Morozova et al., 2010)"
                     /label=TagRFP657
                     /note="derived from mKate"
                     /translation="SELITENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTQRIKVV
                     EGGPLPFAFDILATSFMYGSHTFINHTQGIPDFWKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEAHTEMLYPADGGLEGRTALALKL
                     VGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDYRLERIKEADKETYVEQHEVAVARYCD
                     LPSKLGHKLN"
ORIGIN
        1 tcctccgaca gactgagtcg gccggtcgaa tcaagcttat cgataccgtc gacggatcca
       61 ccggtgccac catgtccgga gccacgcgta gcgagctgat caccgagaac atgcacatga
      121 agctgtacat ggagggcacc gtgaacaacc accacttcaa gtgcacatcc gagggcgaag
      181 gcaagcccta cgagggcacc cagacccaga gaatcaaggt ggtcgagggc ggccctctcc
      241 ccttcgcctt cgacatcctg gctaccagct tcatgtacgg cagtcacacc ttcatcaacc
      301 acacccaggg catccccgac ttctggaagc agtccttccc tgagggcttc acatgggaga
      361 gagtcaccac atacgaagac gggggcgtgc tgaccgctac ccaggacacc agcctccagg
      421 acggctgcct catctacaac gtcaagatca gaggggtgaa cttcccatcc aacggccctg
      481 tgatgcagaa gaaaacactc ggctgggagg cccacaccga gatgctgtac cccgctgacg
      541 gcggcctgga aggcagaacc gcgctggccc tgaagctcgt gggcgggggc cacctgatct
      601 gcaacttcaa gaccacatac agatccaaga aacccgctaa gaacctcaag atgcccggcg
      661 tctactatgt ggactacaga ctggaaagaa tcaaggaggc cgacaaagag acctacgtcg
      721 agcagcacga ggtggctgtg gccagatact gcgacctccc tagcaaactg gggcacaaac
      781 ttaattgaat gcatgagtaa ctgaggatcc gcccctct
//
