LOCUS       sequence-107021-         767 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for RFP657 NHEJ reporter system expression. Allows
            insertion of target sites between the SFFV promoter and RFP657 for 
            detection of fluorescence knockdown. Puromycin resistance..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Heckl D, Kowalczyk MS, Yudovich D, Belizaire R, Puram RV, McConkey 
            ME, Thielke A, Aster JC, Regev A, Ebert BL
  TITLE     Generation of mouse models of myeloid malignancy with combinatorial 
            genetic lesions using CRISPR-Cas9 genome editing.
  JOURNAL   Nat Biotechnol. 2014 Jun 22. doi: 10.1038/nbt.2951.
  PUBMED    24952903
REFERENCE   2  (bases 1 to 767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2014 Jun 22. doi: 10.1038/nbt.2951."
COMMENT     Sequence Label: pL-SFFV.Reporter.RFP657.PAC
FEATURES             Location/Qualifiers
     source          1..767
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(341..361)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 atccagaggt tgattaacag gaacagagcg taaataacgg gaaagtttct taacatgttt
       61 gtcttgtggc aatacacctg aactagtaat tacatatccc taaaaatgta aatgattgcc
      121 ccaccatttt gttttattaa catttaaatg tatacccaaa tcaagaaaaa cagaacaaat
      181 atgggaataa atggcggtaa gatgctcgaa ttcctgcagc ccgggggatc cgtcgacgcg
      241 gccgctttac ttgtacatca ggcaccgggc ttgcgggtca tgcaccaggt gcgcggtcct
      301 tcgggcacct cgacgtcggc ggtgacggtg aagccgagcc gctcgtagaa ggggaggttg
      361 cggggcgcgg aggtctccag gaaggcgggc accccggcgc gctcggccgc ctccactccg
      421 gggagcacga cggcgctgcc cagacccttg ccctggtggt cgggcgagac gccgacggtg
      481 gccaggaacc acgcgggctc cttgggccgg tgcggcgcca ggaggccttc catctgttgc
      541 tgcgcggcca gccgggaacc gctcaactcg gccatgcgcg ggccgatctc ggcgaacacc
      601 gcccccgctt cgacgctctc cggcgtggtc cagaccgcca ccgcggcgcc gtcgtccgcg
      661 acccacacct tgccgatgtc gagcccgacg cgcgtgagga agagttcttg cagctcggtg
      721 acccgctcga tgtggcggtc cggatcgacg gtgtggcgcg tggcggg
//
