LOCUS       sequence-107022-         537 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes gRNA which targets human MBD3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 537)
  TITLE     LentiCRISPR_Hs_MBD3_ex2
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 537)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: LentiCRISPR_Hs_MBD3_ex2
FEATURES             Location/Qualifiers
     source          1..537
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     58..77
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        157..232
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    280..397
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cgtagaaagt aataatttct tgggtagttt gcagttttaa aattatgttt taaaatggac
       61 tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg
      121 aaaggacgaa acaccgcttg cccgtgcgga agtcgagttt tagagctaga aatagcaagt
      181 taaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gcttttttga
      241 attctagatc ttgagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
      301 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
      361 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
      421 agatccactt tggcgccggc tcgagtggct ccggtgcccg tcagtgggca gagcgcacat
      481 cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaaccggt gcctaga
//
