LOCUS       sequence-107034-         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tet-inducible lentiviral vector for ORF expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Meerbrey KL, Hu G, Kessler JD, Roarty K, Li MZ, Fang JE, 
            Herschkowitz JI, Burrows AE, Ciccia A, Sun T, Schmitt EM, Bernardi 
            RJ, Fu X, Bland CS, Cooper TA, Schiff R, Rosen JM, Westbrook TF, 
            Elledge SJ
  TITLE     The pINDUCER lentiviral toolkit for inducible RNA interference in 
            vitro and in vivo.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Mar 1;108(9):3665-70. doi: 
            10.1073/pnas.1019736108. Epub 2011 Feb 9.
  PUBMED    21307310
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1019736108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-03-1- 1"; volume: "108"; issue: "9"; pages: "3665-70"
COMMENT     Sequence Label: pInducer20
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(86..92,93..110,111..116))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(141..265)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(374..398)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(395..433)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    join(466..484,485..507,508..526,527..549,550..568,569..591,
                     592..610,611..633,634..652,653..675,676..694,695..717,
                     718..736)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
ORIGIN
        1 acggtggtat atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc
       61 gacggatcct aactcaaaat ccacacatta tacgagccgg aagcataaag tgtaaagcct
      121 ggggtgccta atgcggccgc catagtgact ggatatgttg tgttttacag tattatgtag
      181 tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc
      241 gttcagcttt tttgtacaaa cttgttgata tctgcagaat tccaccacac tggactagtt
      301 cggggccgcg gaggctggat cggtcccggt gtcttctatg gaggtcaaaa cagcgtggat
      361 ggcgtctcca ggcgatctga cggttcacta aacgagctct gcttatatag gcctcccacc
      421 gtacacgcct acctcgaccc gggtaccgag ctcgactttc acttttctct atcactgata
      481 gggagtggta aactcgactt tcacttttct ctatcactga tagggagtgg taaactcgac
      541 tttcactttt ctctatcact gatagggagt ggtaaactcg actttcactt ttctctatca
      601 ctgataggga gtggtaaact cgactttcac ttttctctat cactgatagg gagtggtaaa
      661 ctcgactttc acttttctct atcactgata gggagtggta aactcgactt tcacttttct
      721 ctatcactga tagggagtgg taaaggatcc tagtccaaac tggatctctg ctgtccctgt
      781 aataaacccg aaaattttga atttttgtaa tttgtttttg
//
