LOCUS       sequence-107045-         790 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for run-off in vitro transcription of Saccharomyces 
            arboricola KBS RNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Dalby AB, Goodrich KJ, Pfingsten JS, Cech TR
  TITLE     RNA recognition by the DNA end-binding Ku heterodimer.
  JOURNAL   RNA. 2013 Jun;19(6):841-51. doi: 10.1261/rna.038703.113. Epub 2013 
            Apr 22.
  PUBMED    23610127
REFERENCE   2  (bases 1 to 790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.038703.113"; journalName: "RNA"; date: "2013-06"; 
            volume: "19"; issue: "6"; pages: "841-51"
COMMENT     Sequence Label: pAD04 S. arboricola telomerase RNA KBS
FEATURES             Location/Qualifiers
     source          1..790
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(105..123)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(130..147)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(130..146)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(139..161)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(355..374)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     474..496
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(534..552)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        620..724
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 agcttgcatg cctgcaggtc gactctagag gatccggatg aagaaaaaag gagcttcatt
       61 tctcaagctc agattttagc gatctacaag tgaaaaaaaa agctcctata gtgagtcgta
      121 ttagaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
      181 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
      241 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgcc tgatgcggta
      301 ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat
      361 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc
      421 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
      481 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt
      541 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
      601 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
      661 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
      721 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
      781 tcctgttttt
//
