LOCUS       sequence-107046-         790 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for run-off in vitro transcription of Saccharomyces 
            paradoxus KBS RNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Dalby AB, Goodrich KJ, Pfingsten JS, Cech TR
  TITLE     RNA recognition by the DNA end-binding Ku heterodimer.
  JOURNAL   RNA. 2013 Jun;19(6):841-51. doi: 10.1261/rna.038703.113. Epub 2013 
            Apr 22.
  PUBMED    23610127
REFERENCE   2  (bases 1 to 790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.038703.113"; journalName: "RNA"; date: "2013-06"; 
            volume: "19"; issue: "6"; pages: "841-51"
COMMENT     Sequence Label: pAD02 S. paradoxus telomerase RNA KBS
FEATURES             Location/Qualifiers
     source          1..790
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(106..124)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(131..148)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(131..147)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(140..162)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(356..375)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     475..497
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(535..553)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        621..725
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 agcttgcatg cctgcaggtc gactctagag gatccggatg aagaaaaaag gagcgccatc
       61 tttcaaactc agattttagc catctataag tagaaaaata acgctcctat agtgagtcgt
      121 attagaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
      181 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
      241 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt
      301 attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa
      361 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
      421 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
      481 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg
      541 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
      601 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
      661 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
      721 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
      781 ttcctgtttt
//
