LOCUS       sequence-107047-         804 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for run-off in vitro transcription of Saccharomyces 
            castellii KBS RNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Dalby AB, Goodrich KJ, Pfingsten JS, Cech TR
  TITLE     RNA recognition by the DNA end-binding Ku heterodimer.
  JOURNAL   RNA. 2013 Jun;19(6):841-51. doi: 10.1261/rna.038703.113. Epub 2013 
            Apr 22.
  PUBMED    23610127
REFERENCE   2  (bases 1 to 804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.038703.113"; journalName: "RNA"; date: "2013-06"; 
            volume: "19"; issue: "6"; pages: "841-51"
COMMENT     Sequence Label: pAD05 S. castellii telomerase RNA KBS
FEATURES             Location/Qualifiers
     source          1..804
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(107..125)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(132..149)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(132..148)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(141..163)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(357..376)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     476..498
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(536..554)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        622..726
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cgccagcttg catgcctgca ggtcgactct agaggatccg gatgaagaaa aaaggagctg
       61 atatagcctc agatataacg tagcgatcta taagtctaaa tcagctccta tagtgagtcg
      121 tattagaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
      181 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
      241 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg
      301 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca
      361 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
      421 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
      481 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc
      541 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
      601 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
      661 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
      721 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
      781 cttcctgttt ttgctcaccc agaa
//
