LOCUS       sequence-107048-         775 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for run-off in vitro transcription of Saccharomyces bayanus 
            KBS RNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Dalby AB, Goodrich KJ, Pfingsten JS, Cech TR
  TITLE     RNA recognition by the DNA end-binding Ku heterodimer.
  JOURNAL   RNA. 2013 Jun;19(6):841-51. doi: 10.1261/rna.038703.113. Epub 2013 
            Apr 22.
  PUBMED    23610127
REFERENCE   2  (bases 1 to 775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.038703.113"; journalName: "RNA"; date: "2013-06"; 
            volume: "19"; issue: "6"; pages: "841-51"
COMMENT     Sequence Label: pAD03 S. bayanus telomerase RNA KBS
FEATURES             Location/Qualifiers
     source          1..775
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(115..133)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(140..157)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(140..156)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(149..171)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(365..384)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     484..506
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(544..562)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        630..734
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gcttgcatgc ctgcaggtcg actctagagg atccggatga agaaaaaagg agcctcccag
       61 gactcagatt ttcaaaattg aaataacgta gcgatctata agtacgacaa ggctcctata
      121 gtgagtcgta ttagaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
      181 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
      241 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgcc
      301 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc
      361 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
      421 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
      481 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
      541 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
      601 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
      661 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
      721 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttt
//
