LOCUS       sequence-107049-         792 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for run-off in vitro transcription of Saccharomyces 
            cerevisiae KBS RNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Dalby AB, Goodrich KJ, Pfingsten JS, Cech TR
  TITLE     RNA recognition by the DNA end-binding Ku heterodimer.
  JOURNAL   RNA. 2013 Jun;19(6):841-51. doi: 10.1261/rna.038703.113. Epub 2013 
            Apr 22.
  PUBMED    23610127
REFERENCE   2  (bases 1 to 792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.038703.113"; journalName: "RNA"; date: "2013-06"; 
            volume: "19"; issue: "6"; pages: "841-51"
COMMENT     Sequence Label: pAD01 S. cerevisiae telomerase RNA KBS
FEATURES             Location/Qualifiers
     source          1..792
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(109..127)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(134..151)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(134..150)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(143..165)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(359..378)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     478..500
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(538..556)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        624..728
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 acgccagctt gcatgcctgc aggtcgactc tagaggatcc ggatgaagaa aaaaggagcg
       61 cattttctaa actcagattt tagccatcta taagtagaaa cacaagctcc tatagtgagt
      121 cgtattagaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
      181 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
      241 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc
      301 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta
      361 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg
      421 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
      481 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc
      541 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
      601 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
      661 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      721 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      781 gccttcctgt tt
//
