LOCUS       sequence-107050-         788 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for run-off in vitro transcription of hairpin from the 
            Kluyveromyces lactis telomerase RNA (nt 229-273).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Dalby AB, Goodrich KJ, Pfingsten JS, Cech TR
  TITLE     RNA recognition by the DNA end-binding Ku heterodimer.
  JOURNAL   RNA. 2013 Jun;19(6):841-51. doi: 10.1261/rna.038703.113. Epub 2013 
            Apr 22.
  PUBMED    23610127
REFERENCE   2  (bases 1 to 788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.038703.113"; journalName: "RNA"; date: "2013-06"; 
            volume: "19"; issue: "6"; pages: "841-51"
COMMENT     Sequence Label: pAD06 K. lactis telomerase RNA hairpin
FEATURES             Location/Qualifiers
     source          1..788
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(102..120)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(127..144)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(127..143)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(136..158)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(352..371)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     471..493
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(531..549)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        617..721
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gcttgcatgc ctgcaggtcg actctagagg atccggatga agaaaaaagg agctgttgtc
       61 atagcatttc taaaatccgt tagaaatact acccaacagc tcctatagtg agtcgtatta
      121 gaattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
      181 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
      241 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt
      301 tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg
      361 ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg
      421 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
      481 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat
      541 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
      601 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
      661 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
      721 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
      781 tgtttttg
//
