LOCUS       sequence-107054-        6312 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression plasmid for HA-tagged mouse Neurexin 1beta AS4(+) 
            [containing the insert at alternatively spliced segment 4 (AS4+)] .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6312)
  AUTHORS   Chih B, Gollan L, Scheiffele P
  TITLE     Alternative splicing controls selective trans-synaptic interactions 
            of the neuroligin-neurexin complex.
  JOURNAL   Neuron. 2006 Jul 20. 51(2):171-8.
  PUBMED    16846852
REFERENCE   2  (bases 1 to 6312)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2006 Jul 20. 51(2):171-8."
COMMENT     Sequence Label: pCAG-HA-Nrxn1beta AS4(+)
FEATURES             Location/Qualifiers
     source          1..6312
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        368..645
                     /label=chicken beta-actin promoter
     intron          647..1664
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1588..1610
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1672..1691
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1909..1935
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(3316..3335)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3381..3436
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3435..3454)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3797..3813)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3797..3813)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3810..3832)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3821..3837
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3845..3851,3852..3869,3870..3875))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3890..3911
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3970..4165
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      4016..4151
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4171..4305
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4208..4227)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4262..4281
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4373..4390)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4544..5132)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4624..4643)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5303..6094,6095..6163))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5926..5945
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6164..6268)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        join(6299..6312,1..366)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
       61 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
      121 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
      181 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
      241 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
      301 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
      361 accatggtcg aggtgagccc cacgttctgc ttcactctcc ccatctcccc cccctcccca
      421 cccccaattt tgtatttatt tattttttaa ttattttgtg cagcgatggg ggcggggggg
      481 gggggggggc gcgcgccagg cggggcgggg cggggcgagg ggcggggcgg ggcgaggcgg
      541 agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa agtttccttt tatggcgagg
      601 cggcggcggc ggcggcccta taaaaagcga agcgcgcggc gggcggggag tcgctgcgac
      661 gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac
      721 tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg ggctgtaatt
      781 agcgcttggt ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc cttgaggggc
      841 tccgggaggg ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg
      901 tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg
      961 cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc
     1021 ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt
     1081 gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc cctccccgag
     1141 ttgctgagca cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc gcggggctcg
     1201 ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg
     1261 ccggggaggg ctcgggggag gggcgcggcg gcccccggag cgccggcggc tgtcgaggcg
     1321 cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag ggacttcctt
     1381 tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc
     1441 gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc cttcgtgcgt
     1501 cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg ggggacggct
     1561 gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta
     1621 gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc
     1681 tggttattgt gctgtctcat cattttggca aagaattcgc ggccgctagc gctaccggac
     1741 tcagatctcg agctcaagct tgggaccatg gcacttccca gatgcatgtg gccaaattat
     1801 gtttggagag ctatgatggc atgtgtggtc cacaggggat ccggtgcccc attgactctc
     1861 tgcttgttgg gatgtttgct acagactttt cacgtactct ctcaaaagta tccatacgac
     1921 gttccggact acgcaggtac cgcatccagt ttgggagcgc accacatcca ccatttccat
     1981 ggcagcagca agcatcattc agtgcctatt gcaatctaca ggtcaccagc atccttgcga
     2041 ggcggacacg ctgggacaac atatatcttt agcaaaggtg gtggacagat tacatataag
     2101 tggcctccca atgaccgccc cagtacacga gcagacaggc tggccatcgg atttagcact
     2161 gtccagaagg aagccgtgtt ggtgcgtgtg gacagttcct caggcctggg tgactacctg
     2221 gagctgcaca tacaccaagg aaaaattgga gttaagttta atgttgggac agatgacatc
     2281 gccatcgaag agtctaatgc aatcattaat gatgggaaat accatgtagt acgtttcaca
     2341 aggagtggtg gcaatgccac gttacaggtg gacagctggc cagctatcga acgctaccct
     2401 gcaggaaaca atgataacga gcgcctggcg attgctagac agcgaattcc atatcgactt
     2461 ggtcgagtag ttgatgaatg gctactcgac aaagggcgtc agctcacaat cttcaatagc
     2521 caagcaacca taataattgg cgggaaagag cagggccagc ccttccaggg tcagctctct
     2581 ggtctttact acaatggctt gaaagttctg aatatggcag cagagaacga tgccaacatc
     2641 gccatagtgg gaaatgtgag gctggtcggt gaagtgcctt cctctatgac aactgagtcg
     2701 acagccactg ccatgcagtc tgagatgtcc acctcaatca tggagaccac cacaaccctg
     2761 gctaccagca cagctagaag aggcaagccc cccacaaagg aacccatcag ccagaccaca
     2821 gatgacatcc ttgtggcctc ggcagagtgt cccagtgacg atgaggacat tgacccctgt
     2881 gagccgagct caggtgggtt agccaacccc accagagtag gtggccgtga accataccca
     2941 ggctctgcag aggtgattcg ggagtccagc agtaccactg gcatggtggt ggggattttc
     3001 gcggcagccg ctctgtgcat cctcatcctc ctctatgcca tgtacaagta ctggaaccgg
     3061 gatgaagggt cgtaccacgt ggatgagagt cgaaactaca tcagtaactc agcacagtcc
     3121 aatggggctg tggtcaagga gaagcaaccc agcagtgcta aaagcgccaa caaaaacaag
     3181 aagaacaagg ataaggagta ttatgtctga ctcgaggtac ccccggggag ctcaattcac
     3241 tcctcaggtg caggctgcct atcagaaggt ggtggctggt gtggccaatg ccctggctca
     3301 caaataccac tgagatcttt ttccctctgc caaaaattat ggggacatca tgaagcccct
     3361 tgagcatctg acttctggct aataaaggaa atttattttc attgcaatag tgtgttggaa
     3421 ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttaa aacatcagaa
     3481 tgagtatttg gtttagagtt tggcaacata tgcccatatg ctggctgcca tgaacaaagg
     3541 ttggctataa agaggtcatc agtatatgaa acagccccct gctgtccatt ccttattcca
     3601 tagaaaagcc ttgacttgag gttagatttt ttttatattt tgttttgtgt tatttttttc
     3661 tttaacatcc ctaaaatttt ccttacatgt tttactagcc agatttttcc tcctctcctg
     3721 actactccca gtcatagctg tccctcttct cttatggaga tccctcgacc tgcagcccaa
     3781 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     3841 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     3901 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     3961 agcggatccg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     4021 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     4081 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     4141 ttttggaggc ctaggctttt gcaaaaagct aacttgttta ttgcagctta taatggttac
     4201 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     4261 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatccgctg cattaatgaa
     4321 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     4381 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     4441 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     4501 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     4561 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     4621 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     4681 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     4741 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     4801 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     4861 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     4921 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     4981 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     5041 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     5101 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     5161 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     5221 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     5281 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     5341 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     5401 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     5461 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     5521 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     5581 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     5641 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     5701 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     5761 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     5821 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     5881 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     5941 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     6001 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     6061 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     6121 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     6181 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     6241 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgggtcga
     6301 cattgattat tg
//
