LOCUS       sequence-107055-         636 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human H2A (with multiple Lysine to Arginine point 
            mutations) tagged with Flag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Mattiroli F, Vissers JH, van Dijk WJ, Ikpa P, Citterio E, Vermeulen 
            W, Marteijn JA, Sixma TK
  TITLE     RNF168 ubiquitinates K13-15 on H2A/H2AX to drive DNA damage 
            signaling.
  JOURNAL   Cell. 2012 Sep 14;150(6):1182-95. doi: 10.1016/j.cell.2012.08.005.
  PUBMED    22980979
REFERENCE   2  (bases 1 to 636)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2012.08"; journalName: "Cell"; date: "2012-09-14- 
            14"; volume: "150"; issue: "6"; pages: "1182-95"
COMMENT     Sequence Label: pCDNA3.1-Flag-H2A K5-9-118-119-125-127-129R
FEATURES             Location/Qualifiers
     source          1..636
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             106..129
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(597..614)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 ctctctggct actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta
       61 tagggagacc caagctggct agcgtttaaa cttaagctta ccatggacta caaggacgac
      121 gatgacaagg tacctaggat ccagtcggga cgtggcaggc agggcggcag agctcgcgcc
      181 aaggccaaga cccgctcttc tcgggccggg cttcagtttc ccgtaggccg agtgcatcgc
      241 ctgctccgca aaggcaacta tgcggagcgg gtcggtgctg gagcgccggt gtacctggcg
      301 gcggtgctgg agtacctgac cgccgagatc ctggagctgg ctggcaacgc ggcccgcgac
      361 aacaagaaga ctcgcatcat cccgcgtcac ctccagctgg ccatccgcaa cgatgaggag
      421 ctcaacaagc ttctgggcaa agtcaccatc gcacagggtg gcgtcctgcc caacatccag
      481 gccgtgctac tgcccaggag gaccgagagc caccacaggg ccaggggcag gtaacagata
      541 tccagcacag tggcggccgc tcgagtctag agggcccgtt taaacccgct gatcagcctc
      601 gactgtgcct tctagttgcc agccatctgt tgtttg
//
