LOCUS       sequence-107056-         637 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human H2A (with multiple Lysine to Arginine point 
            mutations) tagged with Flag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 637)
  AUTHORS   Mattiroli F, Vissers JH, van Dijk WJ, Ikpa P, Citterio E, Vermeulen 
            W, Marteijn JA, Sixma TK
  TITLE     RNF168 ubiquitinates K13-15 on H2A/H2AX to drive DNA damage 
            signaling.
  JOURNAL   Cell. 2012 Sep 14;150(6):1182-95. doi: 10.1016/j.cell.2012.08.005.
  PUBMED    22980979
REFERENCE   2  (bases 1 to 637)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2012.08"; journalName: "Cell"; date: "2012-09-14- 
            14"; volume: "150"; issue: "6"; pages: "1182-95"
COMMENT     Sequence Label: pCDNA3.1-Flag-H2A K5-9-118-119-125-127-129R
FEATURES             Location/Qualifiers
     source          1..637
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(435..458)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(499..518)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(500..518)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(563..614)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(592..612)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 cgagcggccg ccactgtgct ggatatctgt tacctgcccc tggccctgtg gtggctctcg
       61 gtcctcctgg gcagtagcac ggcctggatg ttgggcagga cgccaccctg tgcgatggtg
      121 actttgccca gaagcttgtt gagctcctca tcgttgcgga tggccagctg gaggtgacgc
      181 gggatgatgc gagtcttctt gttgtcgcgg gccgcgttgc cagccagctc caggatctcg
      241 gcggtcaggt actccagcac cgccgccagg tacaccggcg ctccagcacc gacccgctcc
      301 gcatagttgc ctttgcggag caggcgatgc actcggccta cgggaaactg aagcccggcc
      361 cgagaagagc gggtcttggc cttggcgcga gctctgccgc cctgcctgcc acgtcccgac
      421 tggatcctag gtaccttgtc atcgtcgtcc ttgtagtcca tggtaagctt aagtttaaac
      481 gctagccagc ttgggtctcc ctatagtgag tcgtattaat ttcgataagc cagtaagcag
      541 tgggttctct agttagccag agagctctgc ttatatagac ctcccaccgt acacgcctac
      601 cgcccatttg cgtcaatggg gcggagttgt tacgaca
//
