LOCUS       sequence-107062-         584 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human UBA1 tagged with His.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 584)
  AUTHORS   Pichler A, Knipscheer P, Oberhofer E, van Dijk WJ, Korner R, Olsen 
            JV, Jentsch S, Melchior F, Sixma TK
  TITLE     SUMO modification of the ubiquitin-conjugating enzyme E2-25K.
  JOURNAL   Nat Struct Mol Biol. 2005 Mar;12(3):264-9. Epub 2005 Feb 20.
  PUBMED    15723079
REFERENCE   2  (bases 1 to 584)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Struct 
            Mol Biol."; date: "2005-03"; volume: "12(3)"; pages: "264-9. Epub 
            2005 Feb 20"
COMMENT     Sequence Label: pET3a-hUBA1
FEATURES             Location/Qualifiers
     source          1..584
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(10..39)
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
ORIGIN
        1 ggatccttag tgatggtgat ggtgatggtg atggtgatgg cggatggtgt atcggacata
       61 gggaacctcg acatcctcgc cgctctcgtc gttacagcac agctcaagca ccagcgcccg
      121 cacgtggcgg cccagctttc gcttcgacac acggctcaca atctctgtca tcggctgatc
      181 caaccgttcc ttgagcttgg cagctggcat gaagaaggaa tagagcatgg acacgccctg
      241 ggacagcatg gtgatctcta atttgtgctc tgtcttaaaa tagtcgagga actgtttgag
      301 ggtcatctcc tcaccattag gctgcagccc ttgtacctca aagcgatccc acaatgtcca
      361 ctcttggtta tagtactggt gacgtggtgc ggcaaggggt tcagagaaac caaagaaagg
      421 cagggccaag ttgaggaaac cattcttgta ggagtcaagc tgtcggtgcc cctgcacaac
      481 cttgtacagt tccagacaca caaggccaac cacggctgct gtggtcgtgg caatggctgg
      541 gatgatcttc cctgcaatca gcttgctctt gtgccggtct gcag
//
