LOCUS       sequence-107071-         623 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human H2A with K13R tagged with Flag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 623)
  AUTHORS   Mattiroli F, Vissers JH, van Dijk WJ, Ikpa P, Citterio E, Vermeulen 
            W, Marteijn JA, Sixma TK
  TITLE     RNF168 ubiquitinates K13-15 on H2A/H2AX to drive DNA damage 
            signaling.
  JOURNAL   Cell. 2012 Sep 14;150(6):1182-95. doi: 10.1016/j.cell.2012.08.005.
  PUBMED    22980979
REFERENCE   2  (bases 1 to 623)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2012.08"; journalName: "Cell"; date: "2012-09-14- 
            14"; volume: "150"; issue: "6"; pages: "1182-95"
COMMENT     Sequence Label: pCDNA3.1-Flag-H2A K13R
FEATURES             Location/Qualifiers
     source          1..623
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(421..444)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(485..504)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(486..504)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(549..600)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(578..598)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 tgtgctggat atctgttact tgcccttggc cttgtggtgg ctctcggtct tcttgggcag
       61 tagcacggcc tggatgttgg gcaggacgcc accctgtgcg atggtgactt tgcccagaag
      121 cttgttgagc tcctcatcgt tgcggatggc cagctggagg tgacgcggga tgatgcgagt
      181 cttcttgttg tcgcgggccg cgttgccagc cagctccagg atctcggcgg tcaggtactc
      241 cagcaccgcc gccaggtaca ccggcgctcc agcaccgacc cgctccgcat agttgccttt
      301 gcggagcagg cgatgcactc ggcctacggg aaactgaagc ccggcccgag aagagcgggt
      361 cttggccctg gcgcgagctt tgccgccctg cttgccacgt cccgactgga tcctaggtac
      421 cttgtcatcg tcgtccttgt agtccatggt aagcttaagt ttaaacgcta gccagcttgg
      481 gtctccctat agtgagtcgt attaatttcg ataagccagt aagcagtggg ttctctagtt
      541 agccagagag ctctgcttat atagacctcc caccgtacac gcctaccgcc catttgcgtc
      601 aatggggcgg agttgttacg aca
//
