LOCUS       sequence-107073-         806 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent bile acid sensor (BAS) targeted to nucleus that allows 
            spatiotemporal monitoring of bile acid transport in single living 
            cells using FRET.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 806)
  AUTHORS   van der Velden LM, Golynskiy MV, Bijsmans IT, van Mil SW, Klomp LW, 
            Merkx M, van de Graaf SF
  TITLE     Monitoring bile acid transport in single living cells using a 
            genetically encoded Forster resonance energy transfer sensor.
  JOURNAL   Hepatology. 2013 Feb;57(2):740-52. doi: 10.1002/hep.26012. Epub 2013
            Jan 8.
  PUBMED    22899095
REFERENCE   2  (bases 1 to 806)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/hep.26012";
            journalName: "Hepatology"; date: "2013-02"; volume: "57"; issue: 
            "2"; pages: "740-52"
COMMENT     Sequence Label: pCMV NucleoBAS
FEATURES             Location/Qualifiers
     source          1..806
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    127..179
                     /label=MCS
                     /note="multiple cloning site"
     CDS             complement(198..218)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt
       61 ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatca gttatctaga
      121 tccggtggat cccgggcccg cggtaccgtc gactgcagaa ttcgaagctt gagctcgagt
      181 taggtacccg cgtcttctac ctttctcttc ttttttgggg taccgccgct gtcctgcaag
      241 agtctgtgca aaattttatg cttctcggcg gccgcaatac ctgccgccgt cagaaattcc
      301 agcaggacca tgtgatcacg tttttcattc ggatcttttg acagagcgct gaaataactc
      361 aggtaatggt tatctggcag cagcaccgga ccgtcgccga tcggggtgtt ctgctggtaa
      421 tggtccgcca gctggaccga accatcttcg atattgtggc gaattttgaa attcactttg
      481 ataccatttt tctgtttatc tgccataatg taaacattat gggagttata attgtattcc
      541 agtttatggc ccagaatatt gccatcttct ttaaaatcaa tgcctttcag ttcgatgcga
      601 ttgaccaggg tgtcgccttc aaatttcact tcagcgcggg ttttatagtt gccatcatct
      661 ttgaagaaga tggtacgttc ttgcacatag ccttccggca tagccgattt gaagaagtcg
      721 tgctgtttca tgtgatctgg atagcgggca aagcacatca gaccataccc gaacgttgta
      781 accagagtcg gccacggcac cggcag
//
