LOCUS       sequence-107074-         790 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent bile acid sensor (BAS) targeted to nucleus that allows 
            spatiotemporal monitoring of bile acid transport in single living 
            cells using FRET.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 790)
  AUTHORS   van der Velden LM, Golynskiy MV, Bijsmans IT, van Mil SW, Klomp LW, 
            Merkx M, van de Graaf SF
  TITLE     Monitoring bile acid transport in single living cells using a 
            genetically encoded Forster resonance energy transfer sensor.
  JOURNAL   Hepatology. 2013 Feb;57(2):740-52. doi: 10.1002/hep.26012. Epub 2013
            Jan 8.
  PUBMED    22899095
REFERENCE   2  (bases 1 to 790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/hep.26012";
            journalName: "Hepatology"; date: "2013-02"; volume: "57"; issue: 
            "2"; pages: "740-52"
COMMENT     Sequence Label: pCMV NucleoBAS N354K-I372V
FEATURES             Location/Qualifiers
     source          1..790
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    128..180
                     /label=MCS
                     /note="multiple cloning site"
     CDS             complement(199..219)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
       61 tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc agttatctag
      121 atccggtgga tcccgggccc gcggtaccgt cgactgcaga attcgaagct tgagctcgag
      181 ttaggtaccc gcgtcttcta cctttctctt cttttttggg gtaccgccgc tgtcctgcaa
      241 gagtctgtgc aaaattttat gcttctcggc ggccgcaata cctgccgccg tcagaaattc
      301 cagcaggacc atgtgatcac gtttttcatt cggatctttt gacagagcgc tgaaataact
      361 caggtaatgg ttatctggca gcagcaccgg accgtcgccg atcggggtgt tctgctggta
      421 atggtccgcc agctggaccg aaccatcttc gatattgtgg cgaattttga aattcacttt
      481 gataccattt ttctgtttat ctgccataat gtaaacatta tgggagttat aattgtattc
      541 cagtttatgg cccagaatat tgccatcttc tttaaaatca atgcctttca gttcgatgcg
      601 attgaccagg gtgtcgcctt caaatttcac ttcagcgcgg gttttatagt tgccatcatc
      661 tttgaagaag atggtacgtt cttgcacata gccttccggc atagccgatt tgaagaagtc
      721 gtgctgtttc atgtgatctg gatagcgggc aaagcacatc agaccatacc cgaacgttgt
      781 aaccagagtc
//
