LOCUS       sequence-107075-         690 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human H2A with K118R and K119R tagged with Flag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Mattiroli F, Vissers JH, van Dijk WJ, Ikpa P, Citterio E, Vermeulen 
            W, Marteijn JA, Sixma TK
  TITLE     RNF168 ubiquitinates K13-15 on H2A/H2AX to drive DNA damage 
            signaling.
  JOURNAL   Cell. 2012 Sep 14;150(6):1182-95. doi: 10.1016/j.cell.2012.08.005.
  PUBMED    22980979
REFERENCE   2  (bases 1 to 690)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2012.08"; journalName: "Cell"; date: "2012-09-14- 
            14"; volume: "150"; issue: "6"; pages: "1182-95"
COMMENT     Sequence Label: pCDNA3.1-Flag-H2A K118-119R
FEATURES             Location/Qualifiers
     source          1..690
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             112..135
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(603..620)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 gcagagctct ctggctacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac
       61 tcactatagg gagacccaag ctggctagcg tttaaactta agcttaccat ggactacaag
      121 gacgacgatg acaaggtacc taggatccag tcgggacgtg gcaagcaggg cggcaaagct
      181 cgcgccaagg ccaagacccg ctcttctcgg gccgggcttc agtttcccgt aggccgagtg
      241 catcgcctgc tccgcaaagg caactatgcg gagcgggtcg gtgctggagc gccggtgtac
      301 ctggcggcgg tgctggagta cctgaccgcc gagatcctgg agctggctgg caacgcggcc
      361 cgcgacaaca agaagactcg catcatcccg cgtcacctcc agctggccat ccgcaacgat
      421 gaggagctca acaagcttct gggcaaagtc accatcgcac agggtggcgt cctgcccaac
      481 atccaggccg tgctactgcc caggaggacc gagagccacc acaaggccaa gggcaagtaa
      541 cagatatcca gcacagtggc ggccgctcga gtctagaggg cccgtttaaa cccgctgatc
      601 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
      661 cttgaccctg gaaggtgcca ctcccactgt
//
