LOCUS       sequence-107076-         646 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human H2A with K118R and K119R tagged with Flag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 646)
  AUTHORS   Mattiroli F, Vissers JH, van Dijk WJ, Ikpa P, Citterio E, Vermeulen 
            W, Marteijn JA, Sixma TK
  TITLE     RNF168 ubiquitinates K13-15 on H2A/H2AX to drive DNA damage 
            signaling.
  JOURNAL   Cell. 2012 Sep 14;150(6):1182-95. doi: 10.1016/j.cell.2012.08.005.
  PUBMED    22980979
REFERENCE   2  (bases 1 to 646)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2012.08"; journalName: "Cell"; date: "2012-09-14- 
            14"; volume: "150"; issue: "6"; pages: "1182-95"
COMMENT     Sequence Label: pCDNA3.1-Flag-H2A K118-119R
FEATURES             Location/Qualifiers
     source          1..646
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(438..461)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(502..521)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(503..521)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(566..617)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(595..615)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 actcgagcgg ccgccactgt gctggatatc tgttacttgc ccttggcctt gtggtggctc
       61 tcggtcctcc tgggcagtag cacggcctgg atgttgggca ggacgccacc ctgtgcgatg
      121 gtgactttgc ccagaagctt gttgagctcc tcatcgttgc ggatggccag ctggaggtga
      181 cgcgggatga tgcgagtctt cttgttgtcg cgggccgcgt tgccagccag ctccaggatc
      241 tcggcggtca ggtactccag caccgccgcc aggtacaccg gcgctccagc accgacccgc
      301 tccgcatagt tgcctttgcg gagcaggcga tgcactcggc ctacgggaaa ctgaagcccg
      361 gcccgagaag agcgggtctt ggccttggcg cgagctttgc cgccctgctt gccacgtccc
      421 gactggatcc taggtacctt gtcatcgtcg tccttgtagt ccatggtaag cttaagttta
      481 aacgctagcc agcttgggtc tccctatagt gagtcgtatt aatttcgata agccagtaag
      541 cagtgggttc tctagttagc cagagagctc tgcttatata gacctcccac cgtacacgcc
      601 taccgcccat ttgcgtcaat ggggcggagt tgttacgaca ttttgg
//
