LOCUS       sequence-107077-         662 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human H2A (with multiple Lysine to Arginine point 
            mutations) tagged with Flag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Mattiroli F, Vissers JH, van Dijk WJ, Ikpa P, Citterio E, Vermeulen 
            W, Marteijn JA, Sixma TK
  TITLE     RNF168 ubiquitinates K13-15 on H2A/H2AX to drive DNA damage 
            signaling.
  JOURNAL   Cell. 2012 Sep 14;150(6):1182-95. doi: 10.1016/j.cell.2012.08.005.
  PUBMED    22980979
REFERENCE   2  (bases 1 to 662)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2012.08"; journalName: "Cell"; date: "2012-09-14- 
            14"; volume: "150"; issue: "6"; pages: "1182-95"
COMMENT     Sequence Label: pCDNA3.1-Flag-H2A K13-15-118-119R
FEATURES             Location/Qualifiers
     source          1..662
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     24..43
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        24..42
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             84..107
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(575..592)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 actgcttact ggcttatcga aattaatacg actcactata gggagaccca agctggctag
       61 cgtttaaact taagcttacc atggactaca aggacgacga tgacaaggta cctaggatcc
      121 agtcgggacg tggcaagcag ggcggcaaag ctcgcgccag ggccaggacc cgctcttctc
      181 gggccgggct tcagtttccc gtaggccgag tgcatcgcct gctccgcaaa ggcaactatg
      241 cggagcgggt cggtgctgga gcgccggtgt acctggcggc ggtgctggag tacctgaccg
      301 ccgagatcct ggagctggct ggcaacgcgg cccgcgacaa caagaagact cgcatcatcc
      361 cgcgtcacct ccagctggcc atccgcaacg atgaggagct caacaagctt ctgggcaaag
      421 tcaccatcgc acagggtggc gtcctgccca acatccaggc cgtgctactg cccaggagga
      481 ccgagagcca ccacaaggcc aagggcaagt aacagatatc cagcacagtg gcggccgctc
      541 gagtctagag ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag
      601 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
      661 gt
//
