LOCUS       sequence-107079-         953 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast two-hybrid bridge vector expressing PAA-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Wang JT, Smith J, Chen BC, Schmidt H, Rasoloson D, Paix A, Lambrus 
            BG, Calidas D, Betzig E, Seydoux G
  TITLE     Regulation of RNA granule dynamics by phosphorylation of 
            serine-rich, intrinsically disordered proteins in C. elegans.
  JOURNAL   Elife. 2014 Dec 23;4. doi: 10.7554/eLife.04591.
  PUBMED    25535836
REFERENCE   2  (bases 1 to 953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2014-12-23"; volume: "4. doi"; pages: " 10.7554/eLife.04591"
COMMENT     Sequence Label: pJW3.31
FEATURES             Location/Qualifiers
     source          1..953
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             107..127
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    149..173
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttacaacttt ttttacttct tgctcattag aaagaaagca tagcaatcta atctaagttt
       61 tctagaacta gtaaagatgg ataaagcgga attaattccc gagcctccaa aaaagaagag
      121 aaaggtcgaa ttgggtggat cccccatcac aagtttgtac aaaaaagcag gcttaatgtc
      181 ggttgtcgaa gaagccactg acgacgcgct ctacccgatt gccgtgctca ttgacgagct
      241 gcgcaacgaa gatgtcacgt tgcgactgaa cagcattcgc aaactctcca caatcgcttt
      301 ggcgttgggt gtggagcgca cacgcaacga gctcatccaa tttttgaccg acacgattta
      361 cgacgaagat gaggttctgc ttgtgctcgc cgagcaactc ggaaatttca ctccattggt
      421 cggtggtcca gatcatgttc attgtcttct ccttccactt gagaacttgg ccacagtcga
      481 ggagaccgtg gtccgtgaca aagccgttga gagtctccgc aaaatcgccg acaagcacag
      541 ctcggcctca ttggaggagc atttcgtgcc gatgctcaga cgtttggcca ctggagactg
      601 gttcacatcc cgtacctcgg cttgtgggct tttctctgtt gtttatccac gtgtcagtcc
      661 agctatcaag tcggaattga agagcatgtt ccgaaccctg tgccgtgatg acaccccaat
      721 ggttcgccgt gctgctgctg caaagctcgg agagttcgca aaggtgtttg agaagactgc
      781 cgtcatcgag ggtctccact ccagtctcac tgatcttcat gttgatgagc aagattctgt
      841 tcgtttgctc actgtcgagt cagccatcgc tttcggaact ctcctcgata aggctaacaa
      901 gaagaaactc atcgagccaa ttctcattga attgttcgat gacaagtcgt gga
//
