LOCUS       sequence-107082-        9303 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression plasmid for HA-tagged rat Neurexin 1alpha.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9303)
  AUTHORS   Taniguchi H, Gollan L, Scholl FG, Mahadomrongkul V, Dobler E, 
            Limthong N, Peck M, Aoki C, Scheiffele P
  TITLE     Silencing of neuroligin function by postsynaptic neurexins.
  JOURNAL   J Neurosci. 2007 Mar 14;27(11):2815-24.
  PUBMED    17360903
REFERENCE   2  (bases 1 to 9303)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Neurosci."; date: "2007-03-14"; volume: "27(11)"; pages: "2815-24"
COMMENT     Sequence Label: pCAG-HA-rat Nrxn1alpha
FEATURES             Location/Qualifiers
     source          1..9303
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        368..645
                     /label=chicken beta-actin promoter
     intron          647..1664
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1588..1610
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1672..1691
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1909..1935
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(1942..1953)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     primer_bind     complement(6307..6326)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6372..6427
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6426..6445)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6788..6804)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6788..6804)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6801..6823)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6812..6828
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6836..6842,6843..6860,6861..6866))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6881..6902
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        6961..7156
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7007..7142
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7162..7296
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7199..7218)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7253..7272
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7364..7381)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7535..8123)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7615..7634)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8294..9085,9086..9154))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8917..8936
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9155..9259)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        join(9290..9303,1..366)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
       61 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
      121 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
      181 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
      241 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
      301 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
      361 accatggtcg aggtgagccc cacgttctgc ttcactctcc ccatctcccc cccctcccca
      421 cccccaattt tgtatttatt tattttttaa ttattttgtg cagcgatggg ggcggggggg
      481 gggggggggc gcgcgccagg cggggcgggg cggggcgagg ggcggggcgg ggcgaggcgg
      541 agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa agtttccttt tatggcgagg
      601 cggcggcggc ggcggcccta taaaaagcga agcgcgcggc gggcggggag tcgctgcgac
      661 gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac
      721 tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg ggctgtaatt
      781 agcgcttggt ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc cttgaggggc
      841 tccgggaggg ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg
      901 tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg
      961 cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc
     1021 ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt
     1081 gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc cctccccgag
     1141 ttgctgagca cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc gcggggctcg
     1201 ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg
     1261 ccggggaggg ctcgggggag gggcgcggcg gcccccggag cgccggcggc tgtcgaggcg
     1321 cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag ggacttcctt
     1381 tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc
     1441 gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc cttcgtgcgt
     1501 cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg ggggacggct
     1561 gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta
     1621 gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc
     1681 tggttattgt gctgtctcat cattttggca aagaattcgc ggccgctagc gctaccggac
     1741 tcagatctcg agctcaagct tgggaccatg gcacttccca gatgcatgtg gccaaattat
     1801 gtttggagag ctatgatggc atgtgtggtc cacaggggat ccggtgcccc attgactctc
     1861 tgcttgttgg gatgtttgct acagactttt cacgtactct ctcaaaagta tccatacgac
     1921 gttccggact acgcaactag tctggagttc ccaggcgctg agggccagtg gacgcgcttc
     1981 cccaagtgga acgcgtgctg cgagagtgag atgagcttcc agctgaagac acgcagcgcc
     2041 cgcggcctcg tgctctactt cgacgacgag ggcttctgcg acttcctcga gctcatcctg
     2101 acacgcggcg gccgcttgca gctcagcttc tccatcttct gcgccgagcc cgccacgctg
     2161 ttggccgaca cgccagtcaa cgacggcgcc tggcacagcg tgcgcatccg ccgccagttc
     2221 cgcaacacca ctctctacat cgaccgcgct gaggccaagt gggtggaggt caagtccaag
     2281 cgcagggaca tgacggtgtt cagtgggctg ttcgtgggcg gcctgccgcc tgaactgcga
     2341 gccgctgcgc tcaagctcac cctggcctcg gtgcgtgagc gggagccctt caagggctgg
     2401 atccgtgatg tcagggtcaa ctcgtcccag gcgctgccgg tggacggcag cgaggtcaag
     2461 ctggacgaag agccgcccaa tagcgggggc gggagcccgt gcgaggcggg cgatgagggc
     2521 gacggcggcg tgtgcctcaa cgggggcgtg tgctccgtgg tcgacgacca ggccgtgtgt
     2581 gactgctcga ggaccggttt ccgtggcaaa gactgcagcc aaggaaaaga agagtatatt
     2641 gccacattca agggatctga gtacttctgc tacgacttat cccaaaaccc cattcaaagc
     2701 agcagcgacg aaataactct gtcctttaaa actcttcaga ggaatggact gatgcttcac
     2761 acagggaaat cggctgatta tgtcaacctt gccttgaaaa atggagctgt ctctctggtc
     2821 attaatttgg gatcaggggc ctttgaagcg ctagtggagc ccgtgaatgg aaagtttaat
     2881 gataatgcct ggcatgatgt gaaagtcacc aggaatctgc gtcaggtgac aatatcagtg
     2941 gatgggattc ttaccacaac gggctacacg caagaagatt acaccatgct ggggtctgat
     3001 gactttttct atgttggagg cagccccagc acagccgacc ttccagggtc accagtcagt
     3061 aacaacttta tgggctgtct caaagaggtc gtatataaaa ataatgatgt aagactggag
     3121 ttatcgcgac ttgccaagca aggagatcct aagatgaaga tccatggtgt ggtggctttt
     3181 aagtgtgaaa atgtggcaac cttagaccca atcacttttg agaccccgga atctttcatt
     3241 tctttgccta agtggaatgc aaagaaaaca ggctcaatat catttgattt ccgtacaacg
     3301 gagccaaatg gcctcatctt gtttagccat ggcaagccaa gacatcagaa ggatgccaag
     3361 cacccgcaga tgattaaggt tgactttttt gctattgaaa tgctggatgg ccacttgtac
     3421 ctcctcttag acatgggatc aggtactata aaaatcaaag cccttcagaa gaaagtcaat
     3481 gatggagaat ggtatcacgt ggatttccag cgagatggac ggtcaggtac catttctgtc
     3541 aacacacttc gtactcccta cacagctccc ggtgagagtg agatcctaga cctcgatgat
     3601 gagttgtatc tgggtggctt gccagaaaat aaggctggcc tggtcttccc cactgaggtg
     3661 tggactgctc tgctcaacta tggctacgta ggctgcatcc gggatctatt cattgatggc
     3721 cagagcaaag atatccggca gatggcggaa attcagagta ctgctggagt gaagccatcc
     3781 tgctcaaggg agactgcaaa accatgcctt agcaatcctt gcaaaaacaa tggcatgtgc
     3841 agggatggct ggaacagata cgtctgtgat tgctctggaa caggctatct cggcaggtcc
     3901 tgtgagagag aggcaacggt tttgagctac gatgggagta tgtttatgaa aattcagctc
     3961 cctgtggtga tgcacactga ggccgaggac gtttccttac ggttcagatc ccagcgtgcg
     4021 tatggcattc tgatggcgac cacttctaga gactccgcag atacccttcg actggagcta
     4081 gatgcagggc gcgtgaaact cacggtcaat ctagattgta tcaggattaa ctgtaattcc
     4141 agcaaaggtc ctgagaccct ttttgccggc tataacctca atgataatga atggcacaca
     4201 gtacgtgtgg ttcggcgagg aaaaagttta aagctaaccg tggatgatca acaagccatg
     4261 acaggccaaa tggcaggtga tcatacaagg ctggagtttc ataacataga gacaggcatc
     4321 atcacagaac gacgctatct ttcttctgtc ccctccaact tcattgggca cctgcaaagc
     4381 ctcacattta atggaatggc atacattgac ttgtgcaaaa atggtgatat agattattgt
     4441 gaactcaatg ccaggtttgg attcaggaac atcatcgcgg atcctgtcac cttcaagacc
     4501 aaatcaagct atgttgcctt agctacattg caagcctaca cttctatgca tctatttttc
     4561 caattcaaga caacatccct agatggacta attctgtata acagtgggga tggaaatgac
     4621 tttattgtgg ttgaattagt taaagggtac ttacattatg tgtttgactt gggaaatggt
     4681 gctaacctca tcaaagggag ctcaaacaaa ccactcaacg acaatcagtg gcacaatgtg
     4741 atgatttcaa gggacaccag caatctccac acagtaaaga tcgacacaaa aatcacaaca
     4801 caaatcactg cgggagccag aaacttagac ctcaagagtg acttgtatat tggaggagtg
     4861 gctaaagaaa catacaaatc gttgccgaaa ctggtccatg ctaaagaagg ctttcaaggt
     4921 tgcctggcat ctgtggactt gaatggcagg cttccagacc tcatatctga tgctcttttc
     4981 tgcaatgggc aaattgagag aggatgcgaa gggcccagca caacctgtca agaggactca
     5041 tgttcaaatc aaggcgtgtg cttgcagcaa tgggatggct ttagctgtga ctgtagcatg
     5101 acttccttca gtggcccgct ctgcaatgac cctgggacga cgtatatctt tagcaaaggt
     5161 ggtggacaaa ttacatataa gtggcctcct aatgaccgac ccagtacacg agcagacagg
     5221 ctggccatcg ggtttagcac tgttcagaag gaagcagtgt tggtgcgtgt ggacagttcc
     5281 tcaggcctgg gtgactacct tgagctgcac atacaccaag gaaaaattgg agttaaattt
     5341 aatgttggga cagatgacat cgccattgag gagtccaatg caatcattaa tgatggaaaa
     5401 taccatgtag tgcgtttcac gaggagtggt ggcaatgcca cgttacaggt ggacagctgg
     5461 ccagtcatcg aacgataccc tgcagggcgt cagctcacaa tcttcaatag ccaagcaacc
     5521 ataataattg gcgggaaaga gcagggccag cccttccagg gccagctctc tgggctttac
     5581 tacaatggct tgaaagttct gaatatggcg gcagagaacg atgccaacat cgccatagtg
     5641 ggaaatgtga ggctggtcgg tgaagtgcct tcctctatga caactgagtc gacagccact
     5701 gccatgcagt ccgagatgtc cacctcaatc atggagacca ccacaaccct ggctaccagc
     5761 acagctcgac gaggaaagcc ccccacaaag gagcctatca gccagaccac ggatgatatc
     5821 cttgtggcct cggcagagtg tcccagcgac gatgaggaca ttgacccctg tgagccgagc
     5881 tcaggtgggt tagccaaccc cacccgagtg ggcggccgcg aaccataccc aggctcggca
     5941 gaggtgatcc gggagtctag cagtaccact ggcatggtgg tggggattgt cgcagcagca
     6001 gctctgtgca tcctcatcct cctctatgcc atgtacaagt acagaaaccg ggatgaaggc
     6061 tcatatcacg tggatgagag tcgaaactac atcagtaact cagcacagtc caatggggct
     6121 gtggtcaagg agaagcagcc cagcagtgcg aaaagcgcca acaaaaacaa gaagaacaag
     6181 gataaggagt attatgtctg actcgaggta cccccgggga gctcaattca ctcctcaggt
     6241 gcaggctgcc tatcagaagg tggtggctgg tgtggccaat gccctggctc acaaatacca
     6301 ctgagatctt tttccctctg ccaaaaatta tggggacatc atgaagcccc ttgagcatct
     6361 gacttctggc taataaagga aatttatttt cattgcaata gtgtgttgga attttttgtg
     6421 tctctcactc ggaaggacat atgggagggc aaatcattta aaacatcaga atgagtattt
     6481 ggtttagagt ttggcaacat atgcccatat gctggctgcc atgaacaaag gttggctata
     6541 aagaggtcat cagtatatga aacagccccc tgctgtccat tccttattcc atagaaaagc
     6601 cttgacttga ggttagattt tttttatatt ttgttttgtg ttattttttt ctttaacatc
     6661 cctaaaattt tccttacatg ttttactagc cagatttttc ctcctctcct gactactccc
     6721 agtcatagct gtccctcttc tcttatggag atccctcgac ctgcagccca agcttggcgt
     6781 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     6841 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     6901 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagcggatcc
     6961 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     7021 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     7081 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     7141 cctaggcttt tgcaaaaagc taacttgttt attgcagctt ataatggtta caaataaagc
     7201 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     7261 tccaaactca tcaatgtatc ttatcatgtc tggatccgct gcattaatga atcggccaac
     7321 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     7381 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     7441 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     7501 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     7561 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     7621 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     7681 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     7741 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     7801 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     7861 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     7921 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     7981 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     8041 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     8101 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     8161 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     8221 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     8281 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     8341 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     8401 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     8461 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     8521 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     8581 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     8641 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     8701 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     8761 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     8821 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     8881 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     8941 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     9001 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     9061 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     9121 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     9181 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     9241 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgggtcg acattgatta
     9301 ttg
//
