LOCUS       sequence-107091-        7265 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression plasmid of HA-tagged wildtype human Neuroligin 4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7265)
  AUTHORS   Chih B, Afridi SK, Clark L, Scheiffele P
  TITLE     Disorder-associated mutations lead to functional inactivation of 
            neuroligins.
  JOURNAL   Hum Mol Genet. 2004 Jul 15;13(14):1471-7. Epub 2004 May 18.
  PUBMED    15150161
REFERENCE   2  (bases 1 to 7265)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum Mol 
            Genet."; date: "2004-07-15"; volume: "13(14)"; pages: "1471-7. Epub 
            2004 May 18"
COMMENT     Sequence Label: pCAG-HA-NLGN4 WT
FEATURES             Location/Qualifiers
     source          1..7265
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        368..645
                     /label=chicken beta-actin promoter
     intron          647..1664
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1588..1610
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1672..1691
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1812..1838
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(4269..4288)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4334..4389
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4388..4407)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4750..4766)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4750..4766)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4763..4785)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4774..4790
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4798..4804,4805..4822,4823..4828))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4843..4864
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        4923..5118
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      4969..5104
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    5124..5258
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5161..5180)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5215..5234
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5326..5343)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5497..6085)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5577..5596)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6256..7047,7048..7116))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6879..6898
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7117..7221)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        join(7252..7265,1..366)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
       61 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
      121 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
      181 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
      241 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
      301 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
      361 accatggtcg aggtgagccc cacgttctgc ttcactctcc ccatctcccc cccctcccca
      421 cccccaattt tgtatttatt tattttttaa ttattttgtg cagcgatggg ggcggggggg
      481 gggggggggc gcgcgccagg cggggcgggg cggggcgagg ggcggggcgg ggcgaggcgg
      541 agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa agtttccttt tatggcgagg
      601 cggcggcggc ggcggcccta taaaaagcga agcgcgcggc gggcggggag tcgctgcgac
      661 gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac
      721 tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg ggctgtaatt
      781 agcgcttggt ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc cttgaggggc
      841 tccgggaggg ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg
      901 tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg
      961 cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc
     1021 ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt
     1081 gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc cctccccgag
     1141 ttgctgagca cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc gcggggctcg
     1201 ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg
     1261 ccggggaggg ctcgggggag gggcgcggcg gcccccggag cgccggcggc tgtcgaggcg
     1321 cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag ggacttcctt
     1381 tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc
     1441 gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc cttcgtgcgt
     1501 cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg ggggacggct
     1561 gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta
     1621 gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc
     1681 tggttattgt gctgtctcat cattttggca aagaattcgc ggccgctagc accatggctg
     1741 ctggctgcct gctggcctgg acgctgacac tttggcaggc gtggctgatc ctgatcgggc
     1801 cctcgtcgga gtacccatac gacgtcccag actacgctgg tacctatcca gttgtcaaca
     1861 caaattatgg caaaatccgg ggcctaagaa caccgttacc caatgagatc ttgggtccag
     1921 tggagcagta cttaggggtc ccctatgcct caccccccac tggagagagg cggtttcagc
     1981 ccccagaacc cccgtcctcc tggactggca tccgaaatac tactcagttt gctgctgtgt
     2041 gcccccagca cctggatgag agatccttac tgcatgacat gctgcccatc tggtttaccg
     2101 ccaatttgga tactttgatg acctatgttc aagatcaaaa tgaagactgc ctttacttaa
     2161 acatctacgt gcccacggaa gatgatattc atgatcagaa cagtaagaag cccgtcatgg
     2221 tctatatcca tgggggatct tacatggagg gcaccggcaa catgattgac ggcagcattt
     2281 tggcaagcta cggaaacgtc attgtgatca ccattaacta ccgtctggga atactagggt
     2341 ttttaagtac cggtgaccag gcagcaaaag gcaactatgg gctcctggat cagattcaag
     2401 cactgcggtg gattgaggag aatgtgggag cctttggcgg ggaccccaag agagtgacca
     2461 tctttggctc gggggctggg gcctcctgtg tcagcctgtt gaccctgtcc cactactcag
     2521 aaggtctctt ccagaaggcc atcattcaga gcggcaccgc cctgtccagc tgggcagtga
     2581 actaccagcc ggccaagtac actcggatat tggcagacaa ggtcggctgc aacatgctgg
     2641 acaccacgga catggtagaa tgcctgcgga acaagaacta caaggagctc atccagcaga
     2701 ccatcacccc ggccacctac cacatagcct tcgggccggt gatcgacggc gacgtcatcc
     2761 cagacgaccc ccagatcctg atggagcaag gcgagttcct caactacgac atcatgctgg
     2821 gcgtcaacca aggggaaggc ctgaagttcg tggacggcat cgtggataac gaggacggtg
     2881 tgacgcccaa cgactttgac ttctccgtgt ccaacttcgt ggacaacctt tacggctacc
     2941 ctgaagggaa agacactttg cgggagacta tcaagttcat gtacacagac tgggccgata
     3001 aggaaaaccc ggagacgcgg cggaaaaccc tggtggctct ctttactgac caccagtggg
     3061 tggcccccgc cgtggccacc gccgacctgc acgcgcagta cggctccccc acctacttct
     3121 atgccttcta tcatcactgc caaagcgaaa tgaagcccag ctgggcagat tcggcccatg
     3181 gtgatgaggt cccctatgtc ttcggcatcc ccatgatcgg tcccaccgag ctcttcagtt
     3241 gtaacttttc caagaacgac gtcatgctca gcgccgtggt catgacctac tggacgaact
     3301 tcgccaaaac tggtgatcca aatcaaccag ttcctcagga taccaagttc attcacacaa
     3361 aacccaaccg ctttgaagaa gtggcctggt ccaagtataa tcccaaagac cagctctatc
     3421 tgcatattgg cttgaaaccc agagtgagag atcactaccg ggcaacgaaa gtggctttct
     3481 ggttggaact cgttcctcat ttgcacaact tgaacgagat attccagtat gtttcaacaa
     3541 ccacaaaggt tcctccacca gacatgacat catttcccta tggcacccgg cgatctcccg
     3601 ccaagatatg gccaaccacc aaacgcccag caatcactcc tgccaacaat cccaaacact
     3661 ctaaggaccc tcacaaaaca gggcctgagg acacaactgt cctcattgaa accaaacgag
     3721 attattccac cgaattaagt gtcaccattg ccgtcggggc gtcgctcctc ttcctcaaca
     3781 tcttagcttt tgcggcgctg tactacaaaa aggacaagag gcgccatgag actcacaggc
     3841 gccccagtcc ccagagaaac accacaaatg atatcgctca catccagaac gaagagatca
     3901 tgtctctgca gatgaagcag ctggaacacg atcacgagtg tgagtcgctg caggcacacg
     3961 acacactgag gctcacctgc ccgccagact acaccctcac gctgcgccgg tcgccagatg
     4021 acatcccact tatgacgcca aacaccatca ccatgattcc aaacacactg acggggatgc
     4081 agcctttgca cacttttaac accttcagtg gaggacaaaa cagtacaaat ttaccccacg
     4141 gacattccac cactagagta tagctcgagg tacccccggg gagctcaatt cactcctcag
     4201 gtgcaggctg cctatcagaa ggtggtggct ggtgtggcca atgccctggc tcacaaatac
     4261 cactgagatc tttttccctc tgccaaaaat tatggggaca tcatgaagcc ccttgagcat
     4321 ctgacttctg gctaataaag gaaatttatt ttcattgcaa tagtgtgttg gaattttttg
     4381 tgtctctcac tcggaaggac atatgggagg gcaaatcatt taaaacatca gaatgagtat
     4441 ttggtttaga gtttggcaac atatgcccat atgctggctg ccatgaacaa aggttggcta
     4501 taaagaggtc atcagtatat gaaacagccc cctgctgtcc attccttatt ccatagaaaa
     4561 gccttgactt gaggttagat tttttttata ttttgttttg tgttattttt ttctttaaca
     4621 tccctaaaat tttccttaca tgttttacta gccagatttt tcctcctctc ctgactactc
     4681 ccagtcatag ctgtccctct tctcttatgg agatccctcg acctgcagcc caagcttggc
     4741 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     4801 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     4861 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagcggat
     4921 ccgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     4981 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     5041 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     5101 ggcctaggct tttgcaaaaa gctaacttgt ttattgcagc ttataatggt tacaaataaa
     5161 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     5221 tgtccaaact catcaatgta tcttatcatg tctggatccg ctgcattaat gaatcggcca
     5281 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     5341 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     5401 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     5461 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     5521 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     5581 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     5641 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     5701 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     5761 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     5821 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     5881 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     5941 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     6001 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     6061 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     6121 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     6181 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     6241 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     6301 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     6361 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     6421 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     6481 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     6541 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     6601 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     6661 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     6721 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     6781 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     6841 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     6901 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     6961 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     7021 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     7081 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     7141 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     7201 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgggt cgacattgat
     7261 tattg
//
