LOCUS       sequence-107094-        7545 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression plasmid for secreted Fc-fusion of mouse Neurexin 1beta 
            AS4(+) [containing the insert at alternatively spliced segment 4 
            (AS4+)].
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7545)
  AUTHORS   Scheiffele P, Fan J, Choih J, Fetter R, Serafini T
  TITLE     Neuroligin expressed in nonneuronal cells triggers presynaptic 
            development in contacting axons.
  JOURNAL   Cell. 2000 Jun 9. 101(6):657-69.
  PUBMED    10892652
REFERENCE   2  (bases 1 to 7545)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2000 
            Jun 9. 101(6):657-69."
COMMENT     Sequence Label: pcDNA3-Nrxn1beta AS4(+)-Fc
FEATURES             Location/Qualifiers
     source          1..7545
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(40..59)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        231..610
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        611..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     765..785
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     859..878
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        859..877
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(3097..3115)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3098..3115)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3135..3152)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3141..3365
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3411..3839
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3498..3517)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3708..3729
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3848..3868)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3853..4183
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4034..4169
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4096..4115
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4250..5044
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4304..4323)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4914..4933
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5218..5339
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5255..5274)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5309..5328
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5388..5404)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5388..5404)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5401..5423)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5412..5428
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5436..5442,5443..5460,5461..5466))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5481..5502
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5619..5636)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5790..6378)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5870..5889)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6549..7340,7341..7409))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7172..7191
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7410..7514)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gttcccgatc ccctatggtc gactctcagt acaatctgct ctgatgccgc
       61 atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag
      121 caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag
      181 ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt gacattgatt
      241 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
      301 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
      361 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
      421 acgtcaatgg gtggactatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
      481 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
      541 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      601 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      661 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      721 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      781 gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag aacccactgc
      841 ttactggctt atcgaaatta atacgactca ctatagggag acccaagctt accatgtacc
      901 agaggatgct ccggtgcggc gccgatctgg gatcgcccgg gggcggcagt ggcggcggcg
      961 caggggggcg cctggccctg atctggatag tcccgctcac cctcggcggc ctcctaggag
     1021 tggcctgggg ggcatccagt ttgggagcgc accacatcca ccatttccat ggcagcagca
     1081 agcatcattc agtgcctatt gcaatctaca ggtcaccagc atccttgcga ggcggacacg
     1141 ctgggacaac atatatcttt agcaaaggtg gtggacagat tacatataag tggcctccca
     1201 atgaccgccc cagtacacga gcagacaggc tggccatcgg atttagcact gtccagaagg
     1261 aagccgtgtt ggtgcgtgtg gacagttcct caggcctggg tgactacctg gagctgcaca
     1321 tacaccaagg aaaaattgga gttaagttta atgttgggac agatgacatc gccatcgaag
     1381 agtctaatgc aatcattaat gatgggaaat accatgtagt acgtttcaca aggagtggtg
     1441 gcaatgccac gttacaggtg gacagctggc cagttatcga acgctaccct gcaggaaaca
     1501 atgataacga gcgcctggcg attgctagac agcgaattcc atatcgactt ggtcgagtag
     1561 ttgatgaatg gctactcgac aaagggcgtc agctcacaat cttcaatagc caagcaacca
     1621 taataattgg cgggaaagag cagggccagc ccttccaggg tcagctctct ggtctttact
     1681 acaatggctt gaaagttctg aatatggcag cagagaacga tgccaacatc gccatagtgg
     1741 ggaatgtgag gctggtcggt gaagtgagat ctgcggatcc cgagggtgag tacttagctt
     1801 cagcgctcct gcctggacgc atcccggcta tgcagcccca gtccagggca gcaaggcagg
     1861 ccccgtctgc ctcttcaccc ggaggcctct gcccgcccca ctcatgctca gggagagggt
     1921 cttctggctt tttccccagg ctctgggcag gcacaggcta ggtgccccta acccaggccc
     1981 tgcacacaaa ggggcaggtg ctgggctcag acctgccaag agccatatcc gggaggaccc
     2041 tgcccctgac ctaagcccac cccaaaggcc aaactctcca ctccctcagc tcggacacct
     2101 tctctcctcc cagattccag taactcccaa tcttctctct gcagagccca aatcttgtga
     2161 caaaactcac acatgcccac cgtgcccagg taagccagcc caggcctcgc cctccagctc
     2221 aaggcgggac aggtgcccta gagtagcctg catccaggga caggccccag ccgggtgctg
     2281 acacgtccac ctccatctct tcctcagcac ctgaactcct ggggggaccg tcagtcttcc
     2341 tcttcccccc aaaacccaag gacaccctca tgatctcccg gacccctgag gtcacatgcg
     2401 tggtggtgga cgtgagccac gaagaccctg aggtcaagtt caactggtac gtggacggcg
     2461 tggaggtgca taatgccaag acaaagccgc gggaggagca gtacaacagc acgtaccgtg
     2521 tggtcagcgt cctcaccgtc ctgcaccagg actggctgaa tggcaaggag tacaagtgca
     2581 aggtctccaa caaagccctc ccagccccca tcgagaaaac catctccaaa gccaaaggtg
     2641 ggacccgtgg ggtgcgaggg ccacatggac agaggccggc tcggcccacc ctctgccctg
     2701 agagtgaccg ctgtaccaac ctctgtccct acagggcagc cccgagaacc acaggtgtac
     2761 accctgcccc catcccggga tgagctgacc aagaaccagg tcagcctgac ctgcctggtc
     2821 aaaggcttct atcccagcga catcgccgtg gagtgggaga gcaatgggca gccggagaac
     2881 aactacaaga ccacgcctcc cgtgctggac tccgacggct ccttcttcct ctacagcaag
     2941 ctcaccgtgg acaagagcag gtggcagcag gggaacgtct tctcatgctc cgtgatgcat
     3001 gaggctctgc acaaccacta cacgcagaag agcctctccc tgtctccggg taaatgagtg
     3061 cgacggccgc tcgagcatgc atctagaggg ccctattcta tagtgtcacc taaatgctag
     3121 agctcgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
     3181 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
     3241 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
     3301 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
     3361 tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg
     3421 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     3481 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     3541 ctttccccgt caagctctaa atcggggcat ccctttaggg ttccgattta gtgctttacg
     3601 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     3661 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     3721 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     3781 ggggatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta
     3841 attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc aggcaggcag
     3901 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     3961 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     4021 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     4081 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca
     4141 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg
     4201 tatatccatt ttcggatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca
     4261 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
     4321 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
     4381 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc
     4441 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
     4501 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
     4561 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
     4621 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc
     4681 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
     4741 gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct
     4801 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
     4861 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
     4921 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
     4981 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
     5041 ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga
     5101 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
     5161 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac
     5221 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     5281 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     5341 catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt
     5401 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     5461 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     5521 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     5581 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     5641 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     5701 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     5761 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     5821 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     5881 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     5941 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg
     6001 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     6061 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     6121 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     6181 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
     6241 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     6301 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     6361 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     6421 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     6481 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     6541 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     6601 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     6661 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     6721 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     6781 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     6841 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     6901 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     6961 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     7021 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     7081 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     7141 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     7201 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     7261 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     7321 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     7381 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     7441 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     7501 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtc
//
