LOCUS       sequence-107109-         624 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human H2A with K5R and K9R tagged with Flag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Mattiroli F, Vissers JH, van Dijk WJ, Ikpa P, Citterio E, Vermeulen 
            W, Marteijn JA, Sixma TK
  TITLE     RNF168 ubiquitinates K13-15 on H2A/H2AX to drive DNA damage 
            signaling.
  JOURNAL   Cell. 2012 Sep 14;150(6):1182-95. doi: 10.1016/j.cell.2012.08.005.
  PUBMED    22980979
REFERENCE   2  (bases 1 to 624)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2012.08"; journalName: "Cell"; date: "2012-09-14- 
            14"; volume: "150"; issue: "6"; pages: "1182-95"
COMMENT     Sequence Label: pCDNA3.1-Flag-H2A K5-9R
FEATURES             Location/Qualifiers
     source          1..624
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(422..445)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(486..505)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(487..505)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(550..601)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(579..599)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ctgtgctgga tatctgttac ttgcccttgg ccttgtggtg gctctcggtc ttcttgggca
       61 gtagcacggc ctggatgttg ggcaggacgc caccctgtgc gatggtgact ttgcccagaa
      121 gcttgttgag ctcctcatcg ttgcggatgg ccagctggag gtgacgcggg atgatgcgag
      181 tcttcttgtt gtcgcgggcc gcgttgccag ccagctccag gatctcggcg gtcaggtact
      241 ccagcaccgc cgccaggtac accggcgctc cagcaccgac ccgctccgca tagttgcctt
      301 tgcggagcag gcgatgcact cggcctacgg gaaactgaag cccggcccga gaagagcggg
      361 tcttggcctt ggcgcgagct ctgccgccct gcctgccacg tcccgactgg atcctaggta
      421 ccttgtcatc gtcgtccttg tagtccatgg taagcttaag tttaaacgct agccagcttg
      481 ggtctcccta tagtgagtcg tattaatttc gataagccag taagcagtgg gttctctagt
      541 tagccagaga gctctgctta tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt
      601 caatggggcg gagttgttac gaca
//
