LOCUS       sequence-107119-         766 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human Tc3(1–135) bipartite domain tagged with 6xHis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 766)
  AUTHORS   Watkins S, van Pouderoyen G, Sixma TK
  TITLE     Structural analysis of the bipartite DNA-binding domain of Tc3 
            transposase bound to transposon DNA.
  JOURNAL   Nucleic Acids Res. 2004 Aug 10;32(14):4306-12. Print 2004.
  PUBMED    15304566
REFERENCE   2  (bases 1 to 766)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2004-08-10"; volume: "32(14)"; pages: "4306-12. 
            Print 2004"
COMMENT     Sequence Label: pET3CTc3A(1-135)
FEATURES             Location/Qualifiers
     source          1..766
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             416..433
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(487..505)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      501..548
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 agatatacat atgcctcgag gatctgccct ttcggacact gaacgcgctc agctggatgt
       61 tatgaaattg ctcaatgtgt ccctgcatga aatgagtagg aaaatttccc gttctcgaca
      121 ctgtattcgc gtgtatctga aggatccggt gagctacggt acatctaaaa gagctcctcg
      181 tcgcaaagct ctctccgtgc gtgacgaacg aaatgtgatt cgtgctgcct ccaactcctg
      241 taagacggca agagatattc gcaatgagct tcaattgtct gcttcaaaaa ggaccatcct
      301 caatgtcatc aaacgatctg gtgtaatcgt tcgtcagaaa cttcgccctg ctccgttact
      361 ctctgcagac cataaactca agcgattgga atttgctaag aacaatatgg gaacgcacca
      421 tcaccatcac cattaaagat ccggctgcta acaaagcccg aaaggaagct gagttggctg
      481 ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg
      541 gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg
      601 atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag
      661 cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct
      721 agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatat
//
