LOCUS       sequence-107123-         658 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human Tc3(1–135) bipartite domain tagged with 6xHis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Watkins S, van Pouderoyen G, Sixma TK
  TITLE     Structural analysis of the bipartite DNA-binding domain of Tc3 
            transposase bound to transposon DNA.
  JOURNAL   Nucleic Acids Res. 2004 Aug 10;32(14):4306-12. Print 2004.
  PUBMED    15304566
REFERENCE   2  (bases 1 to 658)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2004-08-10"; volume: "32(14)"; pages: "4306-12. 
            Print 2004"
COMMENT     Sequence Label: pET3CTc3A(1-135)
FEATURES             Location/Qualifiers
     source          1..658
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             430..452
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(483..502)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(484..502)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(586..605)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 tggtgatggt gatggtgcgt tcccatattg ttcttagcaa attccaatcg cttgagttta
       61 tggtctgcag agagtaacgg agcagggcga agtttctgac gaacgattac accagatcgt
      121 ttgatgacat tgaggatggt cctttttgaa gcagacaatt gaagctcatt gcgaatatct
      181 cttgccgtct tacaggagtt ggaggcagca cgaatcacat ttcgttcgtc acgcacggag
      241 agagctttgc gacgaggagc tcttttagat gtaccgtagc tcaccggatc cttcagatac
      301 acgcgaatac agtgtcgaga acgggaaatt ttcctactca tttcatgcag ggacacattg
      361 agcaatttca taacatccag ctgagcgcgt tcagtgtccg aaagggcaga tcctcgaggc
      421 atatgtatat ctccttctta aagttaaaca aaattatttc tagagggaaa ccgttgtggt
      481 ctccctatag tgagtcgtat taatttcgcg ggatcgagat ctcgatcctc tacgccggac
      541 gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc tggcgcctat atcgccgaca
      601 tcaccgatgg ggaagatcgg gctcgccact tcgggctcat gagcgcttgt ttcggcgt
//
