LOCUS       sequence-107133-       17876 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human cytoplasmic dynein heavy chain (DYNC1H1) codon optimized for 
            Sf9 expression with a SNAPf tag for fluorescent labeling and a 
            TEV-cleavable His-ZZ tag for purification 
            (His-ZZ-TCS-SNAPf-DYNC1H1)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17876)
  AUTHORS   Schlager MA, Hoang HT, Urnavicius L, Bullock SL, Carter AP
  TITLE     In vitro reconstitution of a highly processive recombinant human 
            dynein complex.
  JOURNAL   EMBO J. 2014 Sep 1;33(17):1855-68. doi: 10.15252/embj.201488792. 
            Epub 2014 Jul 1.
  PUBMED    24986880
REFERENCE   2  (bases 1 to 17876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.15252/embj.201488792"; journalName: "EMBO J"; date: "2014-09-1- 
            1"; volume: "33"; issue: "17"; pages: "1855-68"
COMMENT     Sequence Label: pDyn1
FEATURES             Location/Qualifiers
     source          1..17876
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      190..778
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     679..698
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  913..1136
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(1203..1736)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(1925..1930,1931..1947,1948..1953))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(2024..2057)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        2134..2225
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     2150..2167
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             2285..2308
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             2756..2776
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2798..2818
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2819..3361
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="DKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVEV
                     PAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLKV
                     VKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLAV
                     KEWLLAHEGHRLGKPGLG"
     polyA_signal    17456..17590
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(17493..17512)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     17547..17566
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  17711..17876
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 accggttgac ttgggtcaac tgtcagacca agtttactca tatatacttt agattgattt
       61 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
      121 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
      181 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
      241 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
      301 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
      361 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
      421 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
      481 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
      541 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
      601 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
      661 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
      721 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
      781 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
      841 ctttcctgcg ttatcccctg attgacttgg gtcgctcttc ctgtggatgc gcagatgccc
      901 tgcgtaagcg ggtgtgggcg gacaataaag tcttaaactg aacaaaatag atctaaacta
      961 tgacaataaa gtcttaaact agacagaata gttgtaaact gaaatcagtc cagttatgct
     1021 gtgaaaaagc atactggact tttgttatgg ctaaagcaaa ctcttcattt tctgaagtgc
     1081 aaattgcccg tcgtattaaa gaggggcgtg gccaagggca tgtaaagact atattcgcgg
     1141 cgttgtgaca atttaccgaa caactccgcg gccgggaagc cgatctcggc ttgaacgaat
     1201 tgttaggtgg cggtacttgg gtcgatatca aagtgcatca cttcttcccg tatgcccaac
     1261 tttgtataga gagccactgc gggatcgtca ccgtaatctg cttgcacgta gatcacataa
     1321 gcaccaagcg cgttggcctc atgcttgagg agattgatga gcgcggtggc aatgccctgc
     1381 ctccggtgct cgccggagac tgcgagatca tagatataga tctcactacg cggctgctca
     1441 aacttgggca gaacgtaagc cgcgagagcg ccaacaaccg cttcttggtc gaaggcagca
     1501 agcgcgatga atgtcttact acggagcaag ttcccgaggt aatcggagtc cggctgatgt
     1561 tgggagtagg tggctacgtc tccgaactca cgaccgaaaa gatcaagagc agcccgcatg
     1621 gatttgactt ggtcagggcc gagcctacat gtgcgaatga tgcccatact tgagccacct
     1681 aactttgttt tagggcgact gccctgctgc gtaacatcgt tgctgctgcg taacatcgtt
     1741 gctgctccat aacatcaaac atcgacccac ggcgtaacgc gcttgctgct tggatgcccg
     1801 aggcatagac tgtacaaaaa aacagtcata acaagccatg aaaaccgcca ctgcgccgtt
     1861 accaccgctg cgttcggtca aggttctgga ccagttgcgt gagcgcatac gctacttgca
     1921 ttacagttta cgaaccgaac aggcttatgt caactgggtt cgtgccttca tccgtttcca
     1981 cggtgtgcgt cacccggcaa ccttgggcag cagcgaagtc gccataactt cgtatagcat
     2041 acattatacg aagttatctg taactataac ggtcctaagg tagcgagttt aaacactagt
     2101 atcgattcgc gacctactcc ggaatattaa tagatcatgg agataattaa aatgataacc
     2161 atctcgcaaa taaataagta ttttactgtt ttcgtaacag ttttgtaata aaaaaaccta
     2221 taaatattcc ggattattca taccgtccca ccatcgggcg cggatcctca acatgtctta
     2281 ctatcaccac caccatcacc accatcacgg ttcaatgtgc atggctggcc tcgcccagca
     2341 cgacgaagct gttgacaata agttcaacaa ggaacagcag aacgctttct acgaaatcct
     2401 ccacctgcca aacctcaacg aagaacagcg caacgcattc atccaatcac tgaaggacga
     2461 cccatcccaa tccgcaaacc tgttggccga agcaaagaag ctgaacgacg ctcaggctcc
     2521 caaagtggac aataagttca ataaagagca gcagaacgcc ttctatgaga ttctgcactt
     2581 gcctaacctg aacgaggaac agcgcaatgc tttcatccag tctctgaagg atgatccatc
     2641 acagagcgct aatttgctgg ccgaggccaa gaagctcaat gatgctcaag ctcccaaggt
     2701 ggatgctaac tccgccggaa agtccactga ctacgacatc cctacaactg caagcgagaa
     2761 cctctacttc caaggcgact atgacatccc aactacagag aatctctatt tccagggtga
     2821 caaagactgc gaaatgaagc gcaccaccct ggatagccct ctgggcaagc tggaactgtc
     2881 tgggtgcgaa cagggcctgc accgtatcat cttcctgggc aaaggaacat ctgccgccga
     2941 cgccgtggaa gtgcctgccc cagccgccgt gctgggcgga ccagagccac tgatgcaggc
     3001 caccgcctgg ctcaacgcct actttcacca gcctgaggcc atcgaggagt tccctgtgcc
     3061 agccctgcac cacccagtgt tccagcagga gagctttacc cgccaggtgc tgtggaaact
     3121 gctgaaagtg gtgaagttcg gagaggtcat cagctacagc cacctggccg ccctggccgg
     3181 caatcccgcc gccaccgccg ccgtgaaaac cgccctgagc ggaaatcccg tgcccattct
     3241 gatcccctgc caccgggtgg tgcagggcga cctggacgtg gggggctacg agggcgggct
     3301 cgccgtgaaa gagtggctgc tggcccacga gggccacaga ctgggcaagc ctgggctggg
     3361 tggttcttcc gaacctggcg gcggaggcgg tgaggatgga tctgctggac tggaggttag
     3421 cgctgtccag aacgtggcag atgtttctgt gctccagaag cacctcagga agctggtgcc
     3481 tttgttgttg gaagacggag gcgaagcacc tgctgcactg gaggcagctc tggaagagaa
     3541 gtccgcactc gaacagatgc gcaagttcct gtccgatcct caggtgcaca ccgtgctcgt
     3601 tgagagaagc acattgaaag aggacgtcgg agacgagggt gaggaagaga aggagttcat
     3661 tagctataat atcaacattg acatccacta cggcgtcaag tccaacagcc tcgcctttat
     3721 caagagaact ccagtgatcg acgccgataa gcctgtctct agccaactga gagtgctgac
     3781 cttgtccgaa gacagccctt atgagacttt gcactcattc atcagcaacg cagtggcacc
     3841 tttctttaag tcctacatta gggaaagcgg taaggccgat agggatggag ataagatggc
     3901 accaagcgtg gagaagaaga tcgctgaact cgaaatgggt ctgctgcacc tgcaacagaa
     3961 tattgaaatt cctgagatca gcctccctat tcacccaatg atcactaacg tggctaagca
     4021 gtgttacgaa agaggtgaga aacctaaggt gaccgacttt ggagacaagg tggaggaccc
     4081 aaccttcctg aaccaactgc agtctggagt gaacagatgg attagagaaa ttcagaaagt
     4141 gaccaagctc gaccgcgacc ctgcttccgg caccgctttg caggagatct ctttctggct
     4201 gaacttggaa cgcgctctct accgcatcca ggagaagcgc gagtctcctg aagttctcct
     4261 gactctggac atcctgaaac acggtaagag gttccatgct acagtctcct ttgacactga
     4321 tacaggcttg aagcaggccc tcgaaaccgt gaacgactac aatcccttga tgaaagattt
     4381 ccctctgaac gacctcctgt ccgcaacaga gctggacaag atccgccagg ctctggtggc
     4441 catctttaca cacctccgca agattagaaa cacaaagtat cccatccagc gcgctttgag
     4501 gctggtcgag gccatttcca gagatctgtc ctcacagctg ctcaaagtgt tgggaaccag
     4561 aaagctgatg cacgtcgctt atgaagagtt cgagaaggtg atggtcgcct gctttgaggt
     4621 gttccaaact tgggatgacg agtatgagaa gctgcaggtg ctgctcaggg acatcgtgaa
     4681 gcgcaagaga gaagagaacc tcaagatggt gtggagaatc aaccctgcac acagaaagct
     4741 gcaagccaga ctggatcaga tgcgcaaatt ccgcagacaa cacgagcagc tgagagctgt
     4801 gatcgttagg gttctcaggc ctcaggtgac agccgtggct caacagaatc aaggagaagt
     4861 ccctgaacca caggacatga aggtcgccga agtgctgttt gacgctgccg atgccaacgc
     4921 catcgaagag gtcaacctgg cttatgagaa cgtcaaggag gtggatggcc tcgatgtctc
     4981 caaagaagga accgaggctt gggaagcagc aatgaagagg tatgatgaga gaattgatcg
     5041 cgttgagaca aggatcaccg ctagactcag ggaccagctc ggcaccgcca agaacgctaa
     5101 tgagatgttc cgcatcttct ccagattcaa tgccttgttc gtccgcccac acattcgcgg
     5161 agcaattcgc gaatatcaaa ctcagctgat tcagcgcgtg aaggatgaca tcgaatcctt
     5221 gcacgacaaa ttcaaggtgc aatacccaca gtctcaggct tgtaagatga gccatgtcag
     5281 ggacctccct cccgtgagcg gatcaattat ctgggccaaa caaattgaca ggcagctcac
     5341 cgcctacatg aagagggtgg aggatgtgct gggcaagggc tgggagaatc acgtcgaggg
     5401 acagaagctg aaacaagacg gcgattcttt cagaatgaaa ttgaacaccc aggagatctt
     5461 cgacgattgg gctcgcaaag tccagcaacg caatctcggt gtctccggca ggatcttcac
     5521 tattgagagc acccgcgtcc gcggtaggac tggtaacgtg ttgaaactga aagtcaattt
     5581 cttgcctgag attattactc tgagcaagga ggtgcgcaac ctcaaatggc tcggattcag
     5641 agtcccactc gctattgtga ataaggctca ccaagcaaat cagttgtacc cattcgctat
     5701 ctctctgatc gaatctgtta ggacatacga gagaacatgt gagaaggtcg aggaaagaaa
     5761 tacaatcagc ctgttggttg ccggattgaa gaaggaggtg caagccctca tcgcagaggg
     5821 cattgccctg gtgtgggagt cttacaaatt ggacccttat gttcagagac tcgccgaaac
     5881 tgtcttcaat ttccaggaga aggtggacga cctgttgatt atcgaagaga agatcgactt
     5941 ggaggtccgc tcactggaaa cctgtatgta tgaccacaag accttcagcg agatcctgaa
     6001 cagggtgcag aaggccgtgg acgacctgaa cttgcattcc tactccaacc tccctatctg
     6061 ggtgaataaa ctcgacatgg agatcgaacg catcttggga gttaggctcc aagcaggcct
     6121 gcgcgcctgg actcaggtgc tcttgggaca ggctgaggac aaagccgagg tcgatatgga
     6181 cactgacgct ccacaagttt cccacaagcc cggtggcgaa cccaagatca agaacgtggt
     6241 tcatgagttg agaatcacta accaggtgat ctacctgaat cctccaatcg aagagtgcag
     6301 atacaagctg tatcaagaaa tgtttgcttg gaagatggtt gttctgtcac tgccaaggat
     6361 tcaaagccaa agataccaag ttggagtgca ttacgagctg actgaagaag agaagttcta
     6421 ccgcaacgcc ttgacacgca tgcccgacgg acccgtcgcc ctcgaagaat catactctgc
     6481 tgtgatgggc atcgtgtcag aggtcgaaca atacgttaaa gtgtggctgc agtaccaatg
     6541 tctgtgggat atgcaggccg agaacatcta caaccgcttg ggcgaggacc tgaacaaatg
     6601 gcaagccctg ctcgtgcaga tcaggaaggc cagaggtact ttcgataacg ccgaaaccaa
     6661 gaaggaattc ggtcccgtgg ttatcgacta tggaaaggtg cagtccaaag tgaacctcaa
     6721 atacgactca tggcataaag aggtcctcag caagttcggt cagatgttgg gatctaacat
     6781 gacagagttc cactcccaaa tcagcaagag cagacaggaa ctggaacagc attcagtgga
     6841 caccgccagc acaagcgacg ctgttacctt cattacctac gttcaatctc tgaagcgcaa
     6901 gattaagcag ttcgagaagc aggtcgaatt gtacaggaac ggtcaacgct tgctggagaa
     6961 gcagagattc cagtttcctc caagctggct gtatatcgac aatatcgagg gtgaatgggg
     7021 agccttcaac gatattatga ggcgcaagga ttctgcaatc caacaacaag ttgccaacct
     7081 gcagatgaag atcgtccagg aagatagagc cgtcgaatca aggactacag acctcttgac
     7141 agactgggag aagactaagc ccgtgactgg aaatttgagg ccagaagagg ccctgcaggc
     7201 cctcactatc tatgaaggaa agttcggtcg cctgaaggat gaccgcgaga agtgtgccaa
     7261 ggccaaggaa gcactggagt tgacagacac aggactgctg agcggatcag aggaaagggt
     7321 gcaggttgcc ttggaggaac tccaggattt gaagggcgtc tggtccgagc tgagcaaggt
     7381 ctgggaacaa atcgaccaga tgaaggagca gccttgggtg tccgtgcagc ctcgcaagtt
     7441 gcgccagaat ctcgatgccc tgctgaatca actgaagtcc tttcccgcca ggctgaggca
     7501 gtacgcaagc tacgagttcg tccagcgctt gctgaagggc tatatgaaga tcaacatgct
     7561 ggtgattgaa ctgaagtctg aagctctgaa ggaccgccat tggaaacagt tgatgaagag
     7621 gctccatgtg aactgggtgg tgtcagagct gacactgggt cagatctggg acgtcgattt
     7681 gcagaagaac gaggccatcg tgaaagacgt cctcctggtg gcccagggtg aaatggctct
     7741 ggaagagttt ctgaagcaaa tcagagaggt gtggaataca tatgagctgg acctggttaa
     7801 ttaccagaac aaatgccgcc tgatcagagg atgggatgac ctgtttaaca aggtcaaaga
     7861 acacattaac tccgtcagcg ccatgaagct gagcccatac tataaggtgt tcgaggaaga
     7921 tgctttgtca tgggaggata aactcaatcg cattatggcc ttgttcgacg tgtggatcga
     7981 cgttcaacgc agatgggtgt acctggaggg catctttaca ggctccgccg atattaaaca
     8041 tctcctgccc gtggagactc aaagatttca gtccatctcc accgagttct tggccctcat
     8101 gaagaaggtt tctaagtctc cactcgtcat ggatgttctg aatatccagg gtgtgcagag
     8161 atcattggaa cgcctggctg acttgctggg caagattcag aaggccctgg gcgagtatct
     8221 cgaaagagaa agaagctctt tcccaaggtt ctatttcgtt ggcgacgaag atctcctgga
     8281 aatcatcggt aattcaaaga acgtggccaa gctccagaag cacttcaaga agatgttcgc
     8341 tggcgtgtct agcatcatcc tcaacgagga caactccgtc gtcttgggta tcagctccag
     8401 agaaggtgaa gaggtcatgt ttaagacacc agtgtccatc accgagcacc caaagattaa
     8461 cgaatggctg accttggtgg agaaagagat gcgcgttact ctggcaaagc tgctcgccga
     8521 gtccgttact gaagtggaga tctttggtaa ggccacttct attgacccta atacctacat
     8581 cacttggatt gacaagtacc aggcccagct ggttgttctc tccgcacaaa tcgcttggtc
     8641 tgagaacgtg gaaaccgctc tgtcctccat gggcggaggt ggagatgctg ctccattgca
     8701 ctctgtcctg agcaatgttg aggtgacact gaacgtgctc gctgactctg tgctgatgga
     8761 gcaaccacct ttgcgcaggc gcaaattgga acacctcatc acagaactcg tgcaccagag
     8821 agatgtgacc cgctccttga tcaagagcaa gattgacaat gccaagagct tcgagtggtt
     8881 gtctcagatg aggttctatt tcgacccaaa gcaaactgat gtgctgcagc agctctcaat
     8941 tcagatggcc aatgctaagt tcaactatgg cttcgagtac ttgggtgttc aggataaact
     9001 cgtccaaact ccactcactg accgctgcta tctcactatg acccaggctc tggaggcccg
     9061 cttgggcgga tctccatttg gccctgctgg cactggcaag actgagtctg tgaaggcact
     9121 gggtcaccag ttgggaaggt ttgtgctggt cttcaactgc gacgagactt tcgacttcca
     9181 ggcaatgggt cgcatcttcg ttggcctgtg ccaggtcggt gcttggggct gcttcgatga
     9241 gtttaatcgc ttggaagaaa gaatgctgtc cgccgtgtcc caacaggttc aatgcattca
     9301 agaagctctg agagagcact ctaacccaaa ctacgataag acttcagctc ctatcacctg
     9361 cgagctgctg aacaaacagg tcaaagtctc acccgacatg gctatcttta tcacaatgaa
     9421 tcctggatac gctggaagat ctaacctgcc agataatttg aagaagttgt ttaggagcct
     9481 cgcaatgaca aagcccgacc gccagctgat cgcacaggtg atgttgtact cccagggctt
     9541 tcgcaccgct gaagtcctgg ccaacaagat cgttcctttc tttaagctgt gcgacgaaca
     9601 gctctcttct cagtcccact acgacttcgg cctcagagca ctcaaatctg tgctggtgag
     9661 cgctggtaat gttaagagag agaggattca gaagattaag agggagaaag aagagagagg
     9721 cgaggctgtc gacgaaggcg agatcgccga gaacctgcct gagcaggaga tcctcatcca
     9781 gagcgtctgt gagactatgg ttcccaaact ggttgccgaa gacatcccat tgctgttctc
     9841 tttgctgagc gatgtgttcc caggtgtgca gtaccacaga ggtgaaatga ctgctctccg
     9901 cgaggaactg aagaaggtct gccaggagat gtacttgacc tacggcgacg gcgaagaggt
     9961 cggaggtatg tgggtggaga aggtgctgca gctgtatcag atcactcaga ttaaccacgg
    10021 tctgatgatg gttggaccat ctggttctgg aaagtctatg gcatggagag tcctgctcaa
    10081 ggcactggag agattggagg gcgtggaagg cgtcgcccat atcattgatc ccaaggctat
    10141 cagcaaggac cacctctacg gaaccctgga ccctaatact agggagtgga ccgacggact
    10201 gtttacccac gttctccgca agatcattga ttcagttcgc ggagaactcc agaagaggca
    10261 atggattgtg tttgatggtg acgttgatcc tgagtgggtt gagaatctga actctgttct
    10321 cgacgacaac aagctcctga ccctgcccaa tggcgagcgc ttgtccctgc cacccaatgt
    10381 gagaattatg tttgaagtcc aggacctgaa atacgccacc ttggccaccg tcagcaggtg
    10441 tggtatggtc tggttctctg aagacgtcct ctccaccgac atgatcttca ataatttcct
    10501 cgccagactc agatccatcc ctctcgatga gggtgaagat gaggcccagc gcaggcgcaa
    10561 gggcaaagag gacgaaggcg aagaggctgc ctcaccaatg ctgcagattc aacgcgacgc
    10621 tgccactatc atgcaaccat actttactag caacggtctg gtcacaaagg ctctggagca
    10681 tgcctttcag ttggagcata tcatggatct gactagattg cgctgtctgg gatcactgtt
    10741 cagcatgctg caccaagcct gcagaaacgt ggctcaatat aatgccaacc acccagattt
    10801 cccaatgcag atcgagcagt tggagagata cattcagaga tatttggtct acgccatcct
    10861 gtggtcactg tccggtgact ccagactgaa gatgcgcgca gagctgggag aatacatccg
    10921 caggatcaca accgtgccac tcccaactgc tcccaacatc ccaatcattg actacgaggt
    10981 gtctatttcc ggcgaatgga gcccatggca agctaaagtg ccacaaattg aggtcgagac
    11041 acacaaggtc gccgcacctg atgttgttgt ccctactttg gacactgtca ggcatgaggc
    11101 cctgctgtat acctggttgg ccgagcacaa gccactcgtg ctgtgcggcc ctccaggctc
    11161 aggtaagact atgacactgt ttagcgccct gcgcgccctg cccgacatgg aagtcgtggg
    11221 cctcaacttc tcatcagcta ccacacctga gttgttgctc aagacattcg accactactg
    11281 cgaataccgc aggactccta acggtgtggt gctggctcca gtgcagctgg gcaaatggct
    11341 ggtcctcttc tgtgatgaaa tcaacctgcc agacatggac aagtatggta ctcagagagt
    11401 gatttccttt attagacaaa tggttgaaca tggcggattc tatcgcacct cagatcagac
    11461 ctgggtgaag ctggagagaa tccaattcgt cggtgcttgt aatcctccaa ccgacccagg
    11521 caggaagcct ctgtcacaca ggttcctgcg ccacgttccc gtcgtctatg tcgattaccc
    11581 tggcccagcc tctctcaccc agatctacgg caccttcaac agagcaatgc tcagactgat
    11641 cccttccctg agaacatacg ctgagccact caccgcagcc atggtggagt tctacacaat
    11701 gtcacaagag cgcttcactc aggacaccca acctcactat atctactcac ctcgcgaaat
    11761 gactaggtgg gtgcgcggta tcttcgaggc tttgcgccct ttggagacat tgcccgttga
    11821 aggactcatt aggatctggg ctcacgaggc cctcaggctc ttccaggatc gcctggtcga
    11881 agacgaggaa cgcagatgga ccgatgagaa tattgataca gtcgcactga agcactttcc
    11941 aaacattgat agggagaagg ccatgagccg ccctattctg tactcaaact ggttgtcaaa
    12001 ggactatatc cctgtggatc aagaagaact gagagactac gttaaagcaa gactgaaggt
    12061 gttctacgag gaagaactcg acgttcctct cgttctgttt aacgaggttc tcgatcacgt
    12121 tctccgcatt gaccgcatct ttagacagcc acagggtcat ctcctgctca ttggtgtgag
    12181 cggcgctggt aagaccactc tctcaaggtt cgtcgcctgg atgaacggcc tgtcagtcta
    12241 ccagatcaaa gtgcacagga agtacactgg cgaggatttc gatgaagatc tccgcactgt
    12301 gctgcgcagg tctggatgta agaacgagaa gattgcattc atcatggatg aatctaacgt
    12361 tctcgatagc ggcttcctgg agcgcatgaa cacactgttg gccaacggag aggtccctgg
    12421 cctgtttgaa ggtgatgagt atgcaactct catgacccag tgcaaagaag gtgcacagaa
    12481 agagggcttg atgctggaca gccacgagga attgtataag tggttcacta gccaggtgat
    12541 tcgcaacctc cacgttgtgt ttacaatgaa tccatctagc gagggcctga aggacagggc
    12601 agctacttct cccgccctgt ttaacagatg tgtcctgaat tggtttggcg attggtctac
    12661 cgaggccctg taccaggtcg gcaaggagtt cactagcaag atggacttgg agaagccaaa
    12721 ttacatcgtt cccgactaca tgccagttgt ctacgataag ttgcctcagc ctcccagcca
    12781 tagagaggca atcgtgaact cctgcgtctt tgtccaccaa actctgcacc aggctaacgc
    12841 aaggttggct aagcgcggtg gaaggacaat ggcaattaca cctagacact acttggactt
    12901 tatcaaccat tacgctaact tgttccatga gaagaggtct gaactggaag agcagcagat
    12961 gcacctcaac gtgggtttgc gcaagatcaa ggagacagtg gatcaggttg aagagctgag
    13021 gcgcgacctc cgcatcaagt cacaggagct ggaggtgaag aatgcggccg ccaacgataa
    13081 gctcaagaag atggtcaagg accaacagga agccgagaag aagaaggtta tgtctcagga
    13141 gatccaggag cagctgcaca agcagcagga agtcatcgcc gataagcaga tgtcagtcaa
    13201 agaagatttg gataaggtgg agcctgctgt gatcgaagcc cagaatgctg ttaagtccat
    13261 taagaaacaa catctggtgg aggttcgctc tatggccaac cctcccgcag ctgttaaact
    13321 ggcattggaa tctatctgct tgctgctggg tgagagcact acagattgga aacagattag
    13381 atcaatcatt atgagagaga acttcatccc aacaatcgtt aacttctctg ctgaagagat
    13441 cagcgacgcc atcagggaga agatgaagaa gaactacatg tctaaccctt cctataacta
    13501 cgaaatcgtg aacagagcat ccctggcatg tggtcccatg gtgaaatggg ccatcgcaca
    13561 gctcaactac gccgacatgc tgaagagagt ggagccattg cgcaacgagc tccagaagct
    13621 cgaagatgat gccaaggata accagcagaa ggccaacgaa gtcgaacaga tgattaggga
    13681 cctggaggca tctattgcaa ggtacaagga agaatatgcc gtgctgattt ctgaggccca
    13741 agccattaag gcagacctgg ccgctgtcga ggctaaggtt aacagatcta ccgccctgtt
    13801 gaagagcctg tctgccgagc gcgaacgctg ggagaagacc agcgaaacat tcaagaacca
    13861 aatgtccact attgctggcg attgcctcct gagcgctgca ttcattgcct atgctggcta
    13921 tttcgatcaa cagatgcgcc agaatttgtt caccacatgg tcacatcacc tccagcaggc
    13981 taatattcag ttcaggactg acatcgcccg cactgagtac ctgtccaacg ctgatgagcg
    14041 cctccgctgg caagcctctt ctctccctgc cgatgatctg tgtaccgaga acgctatcat
    14101 gctcaagagg tttaacagat acccactgat tattgatccc tctggacagg caaccgagtt
    14161 cattatgaac gagtataaag atagaaagat tacacgcacc tccttcctcg atgacgcctt
    14221 cagaaagaat ctcgagagcg ccctgaggtt tggtaatcca ctgctggtgc aagatgtcga
    14281 gagctacgat cccgtgctca atcccgtgtt gaacagggag gtcagaagaa ctggtggcag
    14341 agttctgatc actctcggcg atcaggacat tgacctgagc ccttctttcg tcatctttct
    14401 ctcaactcgc gacccaaccg tcgagtttcc tccagacctc tgctcccgcg tgacatttgt
    14461 gaacttcacc gtcactcgct ccagcctgca atcccagtgc ctcaacgagg ttctgaaggc
    14521 agaaagacct gacgtggacg agaagagaag cgatctgttg aagctgcagg gagagtttca
    14581 gctgaggctg agacagttgg agaaatctct gctgcaggct ctcaacgaag tgaagggccg
    14641 cattctggat gacgacacca ttattaccac tctggagaac ctgaagaggg aagctgctga
    14701 agtcaccaga aaggtcgagg aaactgacat cgtcatgcag gaagtcgaga ctgtcagcca
    14761 acagtacctg cccttgtcta cagcctgtag ctcaatctac ttcaccatgg agtccttgaa
    14821 gcaaatccac tttctgtacc agtacagctt gcagttcttc ctcgacatct atcataatgt
    14881 gctctacgag aatccaaacc tcaaaggcgt caccgatcat acacagcgcc tctctattat
    14941 caccaaggac ctgttccagg tggctttcaa tagagtcgct cgcggaatgc tgcatcagga
    15001 tcatatcacc tttgctatgt tgctggcaag gattaagctg aaaggcaccg tcggagagcc
    15061 tacttatgac gcagaatttc agcatttcct gcgcggtaac gaaatcgtcc tctctgctgg
    15121 aagcactccc agaatccagg gtctgactgt ggaacaggcc gaagccgtgg tgaggctgtc
    15181 ctgcctgccc gctttcaagg atctgatcgc taaagtccaa gccgatgagc agttcggtat
    15241 ttggttggac agctcttctc cagagcagac tgttccttac ctgtggtcag aggaaacacc
    15301 agctacacct atcggccagg ctattcacag gttgctgctc attcaggcct ttcgcccaga
    15361 caggctgctg gccatggctc acatgtttgt gagcaccaac ctcggcgaat cattcatgag
    15421 cattatggaa cagcctctgg acttgactca catcgtcggt actgaggtca agccaaacac
    15481 tcctgtgttg atgtgttcag tgcccggcta tgatgcatca ggacacgttg aggatctggc
    15541 cgctgagcag aacacccaaa tcacctccat tgctatcggc tccgccgagg gtttcaatca
    15601 ggccgataag gccatcaaca ctgcagtgaa gtccggtaga tgggtgatgt tgaagaacgt
    15661 tcacctcgct cccggctggt tgatgcaact ggagaagaag ttgcactctt tgcaaccaca
    15721 cgcctgcttc aggctgttcc tgaccatgga gattaatcct aaggtgcctg ttaacctgct
    15781 gagggctggt agaatctttg tcttcgagcc tccacccggt gtcaaggcca acatgctgcg
    15841 cacattctcc agcatcccag tttccaggat ttgtaagtca cctaatgaga gggctcgcct
    15901 gtacttcctc ctggcatggt tccacgcaat tatccaagag cgcttgaggt acgcacctct
    15961 cggatggagc aagaaatacg agttcggtga gtcagatttg cgcagcgctt gtgacaccgt
    16021 ggatacctgg ctggatgata ccgctaaggg tagacagaac atttcaccag ataagatccc
    16081 ttggtccgcc ctgaagacac tcatggctca aagcatctat ggaggaagag ttgacaacga
    16141 atttgaccag aggctcttga atacattctt ggagagattg tttactacca ggtctttcga
    16201 ctctgagttc aagttggcct gcaaggtgga tggacataaa gatattcaga tgccagatgg
    16261 aattaggcgc gaagagtttg tgcagtgggt ggaactcctg cccgatacac agactccaag
    16321 ctggctgggc ttgcccaaca acgctgagag ggtgctgctg actactcaag gtgtcgatat
    16381 gattagcaag atgttgaaga tgcagatgct ggaagacgag gatgatctgg cttacgctga
    16441 aactgagaag aagacccgca ccgattcaac cagcgatgga aggccagctt ggatgaggac
    16501 tttgcatacc accgcctcta actggttgca cctcattcca cagactctga gccacctcaa
    16561 gcgcactgtc gagaatatca aagacccact cttcagattc ttcgagagag aggtgaagat
    16621 gggtgcaaag ttgctccagg acgtcagaca ggacctggcc gacgtggtgc aggtgtgtga
    16681 aggaaagaag aaacaaacta attatctcag gactctcatc aacgagttgg tcaagggcat
    16741 tctgcctcgc tcatggagcc actacaccgt gccagcaggt atgactgtta tccaatgggt
    16801 cagcgatttc agcgaacgca tcaagcagct gcagaatatt tctttggcag ccgcttcagg
    16861 cggcgcaaag gagctgaaga acatccacgt ctgcctcgga ggcctctttg tgcctgaggc
    16921 ctacatcact gctactagac agtacgttgc tcaagctaat agctggtcac tggaggaatt
    16981 gtgtctggag gtgaatgtta ctacttcaca aggagcaaca ctggatgctt gctccttcgg
    17041 agtgactgga ctcaagctcc aaggtgccac ttgcaacaat aacaagctca gcctgtctaa
    17101 tgctatttca accgccctgc cactgacaca actgagatgg gtgaagcaga caaacactga
    17161 gaagaaagcc tccgtggtca ccctgcccgt gtacctcaac ttcaccagag ccgatctgat
    17221 cttcaccgtg gacttcgaga tcgccactaa agaagatccc aggtctttct acgagagagg
    17281 tgtggctgtg ctgtgcaccg agtaatctag agcctgcagt ctcgacaagc ttgtcgagaa
    17341 gtactagagg atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa
    17401 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
    17461 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
    17521 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
    17581 tgtctggatc tgatcactgc ttgagcctag aagatccggc tgctaacaaa gcccgaaagg
    17641 aagctgagtt ggctgctgcc accgctgagc aataactatc ataaccccta gggtataccc
    17701 atctaattgg aaccagataa gtgaaatcta gttccaaact attttgtcat ttttaatttt
    17761 cgtattagct tacgacgcta cacccagttc ccatctattt tgtcactctt ccctaaataa
    17821 tccttaaaaa ctccatttcc acccctccca gttcccaact attttgtccg cccaca
//
