LOCUS       sequence-107145-        1053 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Adenovirus for the expression of gRNAs targeting intron 19 of murine
            Alk and intron 14 of Eml4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Maddalo D, Manchado E, Concepcion CP, Bonetti C, Vidigal JA, Han YC,
            Ogrodowski P, Crippa A, Rekhtman N, de Stanchina E, Lowe SW, Ventura
            A
  TITLE     In vivo engineering of oncogenic chromosomal rearrangements with the
            CRISPR/Cas9 system.
  JOURNAL   Nature. 2014 Dec 18;516(7531):423-7. doi: 10.1038/nature13902. Epub 
            2014 Oct 22.
  PUBMED    25337876
REFERENCE   2  (bases 1 to 1053)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature13902"; journalName: "Nature"; date: "2014-12-18- 
            18"; volume: "516"; issue: "7531"; pages: "423-7"
COMMENT     Sequence Label: Adeno EA
FEATURES             Location/Qualifiers
     source          1..1053
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        161..409
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        431..506
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        597..845
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        867..942
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 gatgttgtag taaatttggg cgtaaccgag taagatttgg ccattttcgc gggaaaactg
       61 aataagagga agtgaaatct gaataatttt gtgttactca tagcgcgtaa tatttgtcta
      121 gggagatccg gtaccgttta aactcgagaa tggcgcatgt gagggcctat ttcccatgat
      181 tccttcatat ttgcatatac gatacaaggc tgttagagag ataattggaa ttaatttgac
      241 tgtaaacaca aagatattag tacaaaatac gtgacgtaga aagtaataat ttcttgggta
      301 gtttgcagtt ttaaaattat gttttaaaat ggactatcat atgcttaccg taacttgaaa
      361 gtatttcgat ttcttggctt tatatatctt gtggaaagga cgaaacaccg tcctggcatg
      421 tctatctgta gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac
      481 ttgaaaaagt ggcaccgagt cggtgctttt ttgttttaga gctagaaata gcaagttaaa
      541 ataaggctag tccgttttta gcgcgtgcgc caattctgca gacaaatggc tctagagagg
      601 gcctatttcc catgattcct tcatatttgc atatacgata caaggctgtt agagagataa
      661 ttggaattaa tttgactgta aacacaaaga tattagtaca aaatacgtga cgtagaaagt
      721 aataatttct tgggtagttt gcagttttaa aattatgttt taaaatggac tatcatatgc
      781 ttaccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgaa
      841 acaccgtttg tcggggtcta ctaggagttt tagagctaga aatagcaagt taaaataagg
      901 ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gcttttttgt tttagagcta
      961 gaaatagcaa gttaaaataa ggctagtccg tttttagcgc gtgcgccaat tctgcagaca
     1021 aatggggtac ccgttacata acttacggta aat
//
